Prevalence and Associated Factors of HIV Testing Among Pregnant Women: A Multilevel Analysis Using the Recent Demographic and Health Survey Data from 11 East African Countries.

AIM: In developing countries, particularly in sub-Saharan Africa, the burden of mother to child transmission (MTCT) of HIV is higher. Although the Joint United Nations Programme on HIV/AIDS (UNAIDS) and other organizations are working to eliminate MTCT, a large number of pregnant women are not screened for HIV in most African countries.
METHODS: The demographic health survey (DHS) used two-stage stratified sampling technique to select the study participants and we appended the most recent DHS done in the 11 East African countries. A weighted sample of 53, 420 women were included. A multilevel logistic regression analysis was used due to the hierarchical structure of the DHS data. To determine whether or not there was a clustering, the Interclass Correlation Coefficient (ICC) and Median Odds Ratio (MOR) were determined. Model comparison was conducted using deviance (-2LL).
RESULTS: The prevalence of HIV testing among pregnant women was 77.56% [95% CI= 77.20%, 77.91%]. In the Multivariable multi-level analysis, variables such as respondent age, wealth index, marital status, educational level, HIV knowledge, HIV stigma indicator, risky sexual activity, women visiting health care facilities, multiple sexual partnership, early sexual initiation, and awareness about MTCT were the individual-level factors that were associated with HIV testing among pregnant women. While residence and community-level education were the community-level factors that were significantly associated with HIV testing.
CONCLUSION: The prevalence of HIV testing and counseling among pregnant women was higher compared to the previous report. Respondent age, wealth index, marital status, educational level, HIV knowledge, HIV stigma indicator, risky sexual activity, women visiting health care facilities, multiple sexual partnership, early sexual initiation, residence, community-level education and awareness about MTCT were the significant determinant of HIV testing.

Background

Globally, in 2015, an average of 1.8 million children under the age of 15 years were living with HIV with children living in Sub-Saharan Africa accounts for the majority of the infection. Despite continuing progress in preventing new HIV infections among children, there are still major challenges in ensuring access to appropriate antiretroviral therapy for children living with HIV. The challenges start with HIV diagnosis among children.1 Although UNAIDS and other organizations are working to eradicate mother-to-child transmission (MTCT) of HIV, only less than 90% of pregnant women are screened for HIV in most African countries.2

More than 90% of infections among children infected with the virus occur through MTCT3 and low HIV test uptake has been considered as a bottleneck for the prevention of mother to child transition (PMTCT).4 HIV infection control depends on the efficacy of new infection prevention approaches and the treatment of people who are already infected.5 In developing countries MTCT of HIV varies from 25% to 48%, which is greater than the report in developed countries.6 One of the key preventive measures for MTCT is HIV screening of all women of childbearing age.7 However, a large number of women are not yet tested for HIV/AIDS.8 HIV/AIDS poses a significant public health burden worldwide.9 HIV testing and counseling services in sub-Saharan Africa is low.9 According to the Joint United Nations Programme, more than 180 000 children were newly infected with HIV in 2017 and mostly through MTCT.10,11

In addition to reducing morbidity and mortality among infected people, early detection of HIV infection is recognized as a critical component of disease spread control.12,13 Several studies indicate that many infected individuals control their behaviors, such as unprotected sex or needle-sharing, that likely transmit the infection to their partners once they are aware of their positive HIV status.14,15

According to different literatures, education level, marital status, place of residence, location, wealth, knowledge of MTCT during pregnancy and having a stigmatizing attitude, knowledge of HIV/AIDS, having risky sexual activity are factors that affect HIV testing among pregnant women.16–19

While HIV testing is a priority for all pregnant women, many women are not screened for HIV during pregnancy.7,17 Insufficient resources, stigma, the attitudes and skills of health workers, and HIV testing strategy are considered to be the key reasons for low HIV testing during pregnancy in sub-Saharan African countries, particularly in eastern Africa.20 So this study aimed to assess the prevalence of HIV testing and associated factor among pregnant women in eastern Africa.

Methods

Data Sources

A secondary data analysis using the pooled data from the most recent Demographic and Health Surveys (DHS) of the 11 East African countries was done. The DHS used a stratified two-stage sampling technique to select the study participants. We used the women’s datasets (individual record file) for this analysis and we included only women who gave birth in the past one year before each survey. Finally, a total weighted sample of 53, 420 women was included for this study. The survey year and the weighted sample taken from each country indicated in Table 1.

Table 1

The Survey Year and Total Weighted Sample for Each Country

Country	Year of Survey	Weighted Sample
Burundi	2016	2987
Ethiopia	2016	5088
Kenya	2014	7967
Comoros	2012	1487
Madagascar	2008	5498
Malawi	2015/16	7519
Mozambique	2011	5455
Rwanda	2352	3589
Uganda	2016	6845
Zambia	2018	4335
Zimbabwe	2013/2014	2651
Total		53,420

Variables of the Study

Outcome Variable

The outcome variable is the uptake of HIV testing during pregnancy (a binary outcome variable coded as yes or no).

Independent Variables

Maternal age, marital status (categorized as never married, married and divorced/widowed/separated), educational status (no education, primary, secondary and higher), wealth index (poorest, poorer, middle, richer and richest), knowledge about HIV/AIDS, having stigma attitude towards people with HIV, risky sexual behavior, age at first sex, multiple sexual partnership, visiting health facility, awareness of MTCT of HIV/AIDS and employment status were the individual level factor included in this study. While community-level education and residency were the community-level factors.

Operational Definitions

Awareness of MTCT of HIV was defined as awareness of women about the possibility of HIV transmission from HIV positive mother to her child, which is generated from three questions knowledge about HIV transmission during breast feeding, during delivery, and pregnancy.

Respondent’s knowledge about HIV/AIDS was created based on six questions: three questions on HIV prevention information and three questions on misconceptions of HIV transmission modes and it was graded as low (score 3), high (score 4–5), or comprehensive (score 6) knowledge.

Having had any sexually transmitted disease, genital sore/ulcer, genital discharge, having at least one sexual partner other than the husband in the last twelve months and multiple lifetime sexual partnership were the five questions asked to determine participants’ risky sexual activities. These were combined into an index of having risky sexual behavior with three categories: “no risk” (score 0), “some risk” (score 1) and “high risk” (score ≥ 2).

More over, six questions reflecting negative attitudes towards people living with HIV/AIDS were used to create a stigma index. This index was graded “no stigma” (score 6), “low stigma” (score 4–5), “moderate stigma” (score 2–3) and “high stigma” (score 1).21

Data Management and Analysis

Before any statistical analysis, the data were weighted to restore the representativeness of the data and to achieve an accurate estimate and standard error. STATA version 14 software was used to extract, recode, and analyze data. A multilevel logistic regression analysis was used due to the hierarchical structure of the DHS data, which violates the independent assumptions of the standard logistic regression model. Four models were incorporated; the null model-a model without explanatory variables, model I-a model with individual-level variables, model II-a model with community-level variables, and model III-a model with individual and community-level variables. The Interclass Correlation Coefficient (ICC) and Median Odds Ratio (MOR) were calculated to determine whether clustering was occurred or not. Deviance (−2LL) and percentage change in variation (PCV) were used to compare models. Among these models, Model III was selected as the best-fit model because it had the lowest deviance. Both bivariable and multivariate multi-level logistic regression were performed. Variables with a p-value of less than 0.2 were considered for multivariable analysis. In the multivariable analysis Variables with a P-value, lower than 0.05 were considered as the statistically significant factors associated with HIV testing among pregnant women.

Results

Socio-Demographic Characteristics of Study Participants

A total of 53,420 reproductive-age women who gave birth in the past one year preceding each survey were included. Around 28% of the study participants were in the age of 20–24 years and more than half (52.61%) of the study participants had primary education. About 46.11% of women were from poor households and more than half (59.67%) of the study participants initiate sex before 20 years of age. Nearly 49% of the study participants had comprehensive knowledge about HIV/AIDS and the majority (85.28%) of the study participants were married. Regarding attitude towards people with HIV/AIDS, more than 95% of the study participant had a negative attitude toward people with HIV. Looking at risky sexual behavior, about 59.67% of the study participants had some risky sexual behavior (Table 2).

Table 2

Sociodemographic Characteristics of the Respondents in Eastern Africa (N=53,420)

Variables		Frequency (%)
Age (years)	15–19	7278 (13.62%)
	20–24	14,750 (27.60%)
	25–29	13,170 (24.64%)
	30–34	9592 (17.95%)
	35 and above	8660 (16.20%)
Highest education level	No education	11,839 (22.15%)
	Primary education	28,123 (52.61%)
	Secondary education	11,451(21.42%)
	Higher education	2039 (3.81%)
Wealth index	Poor	24,647 (46.11%)
	Middle	10,274 (19.22%)
	Rich	18,531 (34.67%)
Risky sexual behavior	No risk	16,892 (31.60%)
	Some risk	31,897 (59.67%)
	High risk	4662 (8.72%)
HIV knowledge	Low knowledge	6049 (11.32%)
	High knowledge	26,112 (48.85%)
	Comprehensive knowledge	21,291 (39.83%)
Marital status	Never married	3736 (6.99%)
	Married/live with partner	45,585 (85.28%)
	Widowed/divorced/separated	4131 (7.73%)
Working status	Not employed	24,907 (46.60%)
	Employed	28,545 (53.40%)
Multiple sexual partner	NoYes	26,168 (48.96%)27,283 (51.04%)
Stigma indicator	No stigma	2051 (3.84%)
	Low stigma	11,065 (20.70%)
	Moderate stigma	22,784 (42.63%)
	High stigma	17,551 (32.84%)
Residence	Urban	11,534 (21.58%)
	Rural	41,917 (78.42%)
Awareness about MTCT	NoYes	1839 (3.44%)51,612 (96.56%)
Age at sex	Before 20 years	40,597 (75.95%)
	At 20 and after years	12,855 (24.05%)
Visit health facility	NoYes	17,039 (31.88%)36,413 (68.12%)
Community-level education	High level of educationLow level of education	25,529 (47.76%)27,922 (52.24%)

Prevalence of HIV Testing Among Pregnant Women in Eastern Africa

The prevalence of HIV testing among pregnant women in eastern Africa was 77.56%[95% CI= 77.20%, 77.91%], which ranges from 8.96% in Madagascar to 99.66% in Rwanda (Figure 1).

Figure 1

The prevalence of HIV testing among pregnant women in eastern Africa.

Random Effect Model and Model Fitness

ICC, MOR and PCV were used to assess the random-effect model. The ICC value of 0.27 in the null model shows that 27% of the overall variation in HIV testing among pregnant women was due to variations between clusters/communities. Besides, the highest MOR value of 2.72 suggests that substantial clustering of HIV testing among pregnant women has occurred. Also, the highest PCV (0.35) in the final model showed that both the individual and community-level variables explained about 35% of the variation in HIV testing. The final model (model III), which incorporates both individual and community level variables, was the best fitted model since it had the lowest deviance (Table 3).

Table 3

Random Effect Model and Model Fitness for the Assessment of HIV Testing Among Pregnant Women in Eastern Africa

Parameter	Null Model	Model I	Model II	Model III
ICC	0.27	0.33	0.23	0.33
PCV	Reff	0.32	0.17	0.35
MOR	2.72	5.57	2.61	3.4
Model comparison
Log likelihood	−26,417.022	−17,451.431	−25,784.047	−17,367.74
Deviance	52,834.044	34,902.862	50,568.094	34,735.48

Factors Associated with HIV Testing Among Pregnant Women in Eastern Africa

We consider the final model for determining factors associated with HIV testing since it had the lowest deviance. In the multivariable multi-level analysis variables such as respondent age, residence, wealth index, marital status, educational level, HIV knowledge, HIV stigma indicator, risky sexual activity, women visiting health care facilities, multiple sexual partnership, early sex initiation, awareness about MTCT and community level of education were significantly associated with HIV testing among pregnant women. Pregnant women who were aged between 20 and 24 [AOR=1.51: 95% CI: 1.37, 1.65], 25–29 [AOR=1.52: 95% CI; 1.37, 1.68], 30–34 [AOR=1.58: 95% CI; 1.42, 1.76], and 35 and above years (AOR=1.45: 95% CI; 1.30, 1.61) had higher odds of being tested for HIV compared with women aged 15–19 years of age. Pregnant women who had visited a health facility in the last 12 months had 2.56 (AOR=2.56: 95% CI; 2.40, 2.72) times higher odds of being tested for HIV. Regarding educational status, the chances of HIV testing were 2.57 (AOR=2.57; 95% CI; 2.40, 2.75), 2.47 (AOR=2.47; 95% CI; 2.15, 2.62) and 3.77 (AOR= 3.77; 95% CI; 2.94, 4.83) times higher for women with primary, secondary and higher education, respectively, compared to women without formal education. Considering household wealth status, women from the rich household (AOR=1.17: 95% CI; 1.08, 1.27) had higher chances of being tested for HIV compared with women from poor households. Widowed/divorced/separated women had a 38% (AOR=0.62: 95% CI; 0.53, 0.74) lower chance of being tested for HIV compared with never-married women. A woman who had no sexual partner other than her husband was less likely to be tested for HIV (AOR=0.20: 95% CI; 0.18, 0.22). Women who had initiated sex at an older age had 1.49 (AOR=1.49: 95% CI; 1.38, 1.60) times higher odds of being tested for HIV compared with those who initiate sex at an early age. Being rural dweller had 39% (AOR=0.61: 95% CI; 0.56, 0.67) lower chances of being tested for HIV/AIDS compared with their counterparts. Regarding stigma attitude, there were 9.55 (AOR=9.55: 95% CI; 8.92, 10.22), 21.91 (AOR=21.91: 95% CI; 19.79, 24.26) and 26.07 (AOR=26.07: 95% CI; 20.62, 32.97) times higher chances of being tested for HIV testing for women with moderate, low stigma score and those with no stigmatized attitude, respectively, compared to women with higher stigmatized attitude. Importantly, women with higher (AOR=1.09: 95% CI; 1.01, 1.18) and comprehensive (AOR=3.31: 95% CI; 3.01, 3.65) knowledge about HIV/AIDS were more likely to be tested for HIV compared to those with low knowledge. Women with risky sexual behavior were more likely to be tested for HIV compared to women with no risky sexual behavior. Women who were aware of MTCT of HIV/AIDS were more likely to be tested for HIV/AIDS (AOR=1.29: 95% CI; 1.12, 1.48). Pregnant women from communities with higher community level of education had 1.88 (AOR=1.88: 95% CI; 1.58, 2.25) times higher odds of being tested for HIV/AIDS compared with those from communities with lower community level of education. Regarding employment status, employed women had a lower chance of being tested for HIV/AIDS compared with unemployed women (AOR=0.78: 95% CI; 0.74, 0.83) (Table 4).

Table 4

The Bivariable and Multivariable Multilevel Binary Logistic Regression Analysis of Factors Associated with HIV Testing Among Pregnant Women in Eastern Africa

Variables		Ever Tested for HIV		COR(95% CI)	AOR(95% CI)
		No	Yes
Respondent age	15–19	1922	5356	1	1
	20–24	3000	11,744	1.48(1.38, 1.59)	1.51(1.37, 1.66)*
	25–29	2761	10,402	1.41(1.31, 1.51)	1.52(1.37, 1.68)*
	30–34	2140	7435	1.41(1.31, 1.52)	1.58(1.42, 1.76)*
	35 and above	2165	6495	1.19(1.10, 1.29)	1.45(1.30, 1.61)*
Visiting health facility	No	5943	11,074	1	1
	Yes	6043	30,358	3.53(3.36, 3.70)	2.56(2.40, 2.72)*
Highest educational level	No education	5192	6633	1	1
	Primary education	5449	22,659	3.59(3.40, 3.78)	2.57(2.40, 2.75)*
	Secondary education	1259	10,189	4.69(6.03, 6.98)	2.47(2.15, 2.62)*
	Higher education	88	1951	15.36(12.48, 18.91)	3.77(2.94, 4.83)*
Wealth status	Poor	6925	17,699	1	1
	Middle	2459	7811	1.49(1.40, 1.58)	1.06(0.98, 1.14)*
	Rich	2603	15,922	2.84(2.69. 3.01)	1.17(1.08, 1.27)*
Marital status	Never married	385	3351	1	1
	Married	10,609	34,945	0.45(0.41, 0.51)	0.92(0.80, 1.06)
	Widowed/divorced/separated	994	3136	0.41(0.36, 0.47)	0.62(0.53, 0.74)*
Multiple sexual partner	No	5340	20,806	0.94(0.89, 0.98)	0.20(0.18, 0.22)*
	Yes	6648	20,625	1	1
HIV knowledge	Low knowledge	2473	3571	1	1
	Higher knowledge	7976	18,122	1.49(1.39. 1.58)	1.09(1.01, 1.18)*
	Comprehensive knowledge	1539	19,738	9.48(8.75, 10.28)	3.31(3.01, 3.65)*
Stigma indicator	Higher stigma	84201	9110	1	1
	Moderate stigma	2697	20,075	12.22(11.51, 12.98)	9.55(8.92, 10.22)*
	Low stigma	809	10,256	26.09 (23.76, 28.64)	21.91(19.79, 24.26)*
	No stigma	61	1990	35.51(28.36, 44.46)	26.07(20.62, 32.97)*
Residence	Urban	1108	10,418	1	1
	Rural	10,878	31,014	0.36(0.34, 0.39)	0.61(0.56, 0.67)*
Working statues	Not employed	5392	19,486	1	1
	Employed	6595	21,946	0.96(0.91, 1.00)	0.78(0.74, 0.83)*
Risky sexual behavior	Higher risk	525	4137	2.79(2.52, 3.10)	8.66(7.37, 10.17)*
	Some risk	7149	24,720	1.19(1.13, 1.24)	4.61(4.16, 5.11)*
	No risk	4313	12,575	1	1
Age at sex	Before 20 years	9498	31,085	1	1
	At 20 and above years	2489	10,347	1.32(1.25, 1.39)	1.49(1.38, 1.60)*
Awareness of MTCT	No	550	1286	1	1
	Yes	11,438	40,145	1.62(1.45, 1.81)	1.29(1.12, 1.48)*
Community level education	Low	6849	18,666	1	1
	High	5139	22,766	2.92(2.54, 3.36)	1.88(1.58, 2.25)*

Note: *p-value≤0.05.

Discussion

HIV testing during pregnancy provides an opportunity for the prevention of mother-to-child transmission and to initiate lifelong antiretroviral treatment for HIV-positive women before the disease progress to AIDS. This study showed that 77.56%[95% CI=77.20%, 77.91%] of women who gave birth in the past one year before each survey got HIV test. It was greater than studies done in Ethiopia,16 Nigeria,22 and Gambia.23 This finding was smaller than the prevalence of HIV testing reported in Spain24 and Malawi.2 The discrepancy might be due to the difference in the study period, the study population, and the availability and accessibility of maternal health services between countries.

In the multi-level multivariate analysis, age of respondent, educational level, household wealth status, visiting health facility, residence, age at sex, occupational status, knowledge about HIV/AIDS, stigmatized attitude about people with HIV/AIDS, risky sexual behavior, having awareness about MTCT, having multiple sexual partners, marital status and community-level of women education were significantly associated with HIV testing among pregnant women in eastern Africa. Being older had higher odds of testing for HIV compared with young aged women. This finding is supported by a study conducted in Malawi.2 This may be attributable to young individuals might have a low engagement of sexual behaviors, therefore perceive themselves as having a lower risk of HIV, which in turn contributes to low uptake of HIV testing and counseling.2

In this study women with primary and higher education had higher odds of HIV testing compared with women without formal education, which is supported by studies conducted in Ethiopia21,25 and Spain.24 The reason for this is that education can improve HIV-related knowledge and improves income among women that in turn increases maternal health service use.25 Also, educated women might have higher levels of exposure to HIV/AIDS-related information, better knowledge regarding the advantages of HIV testing, as well as ability to make good decisions to go for HIV testing.24

Women from rural resident had less chance of HIV testing compared with those of urban residents and this is supported by a previous study in Ethiopia.16 This might be justified as in most developing countries the health facilities were not accessible to the rural population and this decreases their chance of getting HIV counseling and testing.26 Pregnant women with some and higher risky sexual behavior had higher odds of being tested for HIV/AIDS compared with women with no risky sexual behavior. This finding was supported by studies conducted elsewhere.18,21,27 Individuals with risky sexual behaviors live under persistent fear and uncertainty about their serostatus and are usually suspicious and worried that they might have infected with HIV, this could have urged them to develop habits of seeking voluntary counseling and testing service.28 Concerning HIV/AIDS knowledge, pregnant women having higher and comprehensive knowledge about HIV had higher chances of HIV testing compared to women with low knowledge. This finding was supported by different studies.18,21 This might be justified by people with a good understanding of HIV/AIDS including its transmission and preventive measures helps them to seek HIV testing voluntarily.29 Looking at marital status, widowed/divorced/separated women had lower odds of being tested for HIV compared with unmarried women. This finding was supported by another study conducted in Ethiopia.21 This might be associated with those populations were less likely to visit the health facility and to have voluntary counseling and testing unlike those of married women.21 Regarding household wealth status, a woman in the high socioeconomic categories was more likely to be tested for HIV than women from poor household. This finding was consistent with other studies.16,29 This can be justified as women with higher socioeconomic status have a better educational level and are economically privileged to seek and access health services including voluntary counseling and testing services than those in the lower socioeconomic class.21

The study at hand also revealed that women who had visited a health facility in the past 12 months were more likely to be tested for HIV/AIDS, which was in agreement with the study done in Ethiopia.25 This might be as women who had visited the health facility were advised and supported by the health workers to have voluntary counseling and testing.25 Similarly, pregnant women having awareness about mother-to-child transmission of HIV had a higher chance of being tested for HIV/AIDS compared with their counterparts. This finding was supported by studies in Ethiopia.25,29

In this study women with multiple sexual partnership were more likely to be tested for HIV/AIDS compared with their counterparts. A similar finding was reported from a study in Ethiopia.25 This might be due to the fact that women with multiple sexual partnership had a higher perceived risk of acquiring HIV, which increases their motive to be tested.30

Moreover, women who had initiated sex after 20 years of age had higher odds of HIV testing compared with women who initiated sex before the age of 20 years. This was supported by the study conducted in sub-Saharan Africa.31 This might be explained as early age at first sexual intercourse is associated with a higher risk of acquiring different sexually transmitted disease and risky sexually behavior that may leads to higher risk for HIV infection, which indirectly enforce them to know their HIV status.32

Strength and Limitation of the Study

First, the study was based on weighted nationally representative data from 11 eastern African countries with large sample size. Also, we used the multilevel analysis to accommodate the hierarchical nature of the DHS data to get reliable standard error and estimate. Moreover, since it is based on the national survey data the study has the potential to give insight for policy-makers and program planners to design appropriate intervention strategies both at national and regional levels. The potential limitation of the study was that the DHS survey was based on respondents’ self-report, this might have the possibility of recall bias. Besides, since this study was based on cross-sectionally conducted DHS data, it is difficult to show the temporal relationship between HIV testing and independent variables.

Conclusion

The prevalence of HIV testing among pregnant women was higher in eastern Africa compared with the report from different studies. Different socioeconomic and behavioral characteristics were associated with HIV testing. Age of respondent, wealth status, educational status, marital status, awareness about MTCT of HIV/AIDS, HIV knowledge, stigma indicator, risky sexual behavior, women who visit a health facility, multiple sexual partnership, age at first sex and employment status were the individual-level factors that were significantly associated with HIV testing among pregnant women in eastern Africa. While residence and community-level education were the community-level factors that affect HIV testing among pregnant women. Therefore, giving special attention for those group of women who are at higher risks of not having HIV testing such as those from rural areas and uneducated women could increase HIV/AIDS testing.