Mathias Frontini1, Arnaud Boisnard2, Julien Frouin3, Malika Ouikene4, Jean Benoit Morel1, Elsa Ballini5. 1. BGPI, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, France. 2. Centre Français du Riz, Arles, France. 3. AGAP, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, France. 4. Groupe de Valorisation des Produits Agricoles (GVAPRO), Alger, Algeria. 5. BGPI, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, France. elsa.ballini@supagro.fr.
Abstract
BACKGROUND: Nitrogen fertilization is known to increase disease susceptibility, a phenomenon called Nitrogen-Induced Susceptibility (NIS). In rice, this phenomenon has been observed in infections with the blast fungus Magnaporthe oryzae. A previous classical genetic study revealed a locus (NIS1) that enhances susceptibility to rice blast under high nitrogen fertilization. In order to further address the underlying genetics of plasticity in susceptibility to rice blast after fertilization, we analyzed NIS under greenhouse-controlled conditions in a panel of 139 temperate japonica rice strains. A genome-wide association analysis was conducted to identify loci potentially involved in NIS by comparing susceptibility loci identified under high and low nitrogen conditions, an approach allowing for the identification of loci validated across different nitrogen environments. We also used a novel NIS Index to identify loci potentially contributing to plasticity in susceptibility under different nitrogen fertilization regimes. RESULTS: A global NIS effect was observed in the population, with the density of lesions increasing by 8%, on average, under high nitrogen fertilization. Three new QTL, other than NIS1, were identified. A rare allele of the RRobN1 locus on chromosome 6 provides robust resistance in high and low nitrogen environments. A frequent allele of the NIS2 locus, on chromosome 5, exacerbates blast susceptibility under the high nitrogen condition. Finally, an allele of NIS3, on chromosome 10, buffers the increase of susceptibility arising from nitrogen fertilization but increases global levels of susceptibility. This allele is almost fixed in temperate japonicas, as a probable consequence of genetic hitchhiking with a locus involved in cold stress adaptation. CONCLUSIONS: Our results extend to an entire rice subspecies the initial finding that nitrogen increases rice blast susceptibility. We demonstrate the usefulness of estimating plasticity for the identification of novel loci involved in the response of rice to the blast fungus under different nitrogen regimes.
BACKGROUND:Nitrogen fertilization is known to increase disease susceptibility, a phenomenon called Nitrogen-Induced Susceptibility (NIS). In rice, this phenomenon has been observed in infections with the blast fungus Magnaporthe oryzae. A previous classical genetic study revealed a locus (NIS1) that enhances susceptibility to rice blast under high nitrogen fertilization. In order to further address the underlying genetics of plasticity in susceptibility to rice blast after fertilization, we analyzed NIS under greenhouse-controlled conditions in a panel of 139 temperate japonica rice strains. A genome-wide association analysis was conducted to identify loci potentially involved in NIS by comparing susceptibility loci identified under high and low nitrogen conditions, an approach allowing for the identification of loci validated across different nitrogen environments. We also used a novel NIS Index to identify loci potentially contributing to plasticity in susceptibility under different nitrogen fertilization regimes. RESULTS: A global NIS effect was observed in the population, with the density of lesions increasing by 8%, on average, under high nitrogen fertilization. Three new QTL, other than NIS1, were identified. A rare allele of the RRobN1 locus on chromosome 6 provides robust resistance in high and low nitrogen environments. A frequent allele of the NIS2 locus, on chromosome 5, exacerbates blast susceptibility under the high nitrogen condition. Finally, an allele of NIS3, on chromosome 10, buffers the increase of susceptibility arising from nitrogen fertilization but increases global levels of susceptibility. This allele is almost fixed in temperate japonicas, as a probable consequence of genetic hitchhiking with a locus involved in cold stress adaptation. CONCLUSIONS: Our results extend to an entire rice subspecies the initial finding that nitrogen increases rice blast susceptibility. We demonstrate the usefulness of estimating plasticity for the identification of novel loci involved in the response of rice to the blast fungus under different nitrogen regimes.
Authors: S Ashkani; M Y Rafii; M Shabanimofrad; A Ghasemzadeh; S A Ravanfar; M A Latif Journal: Crit Rev Biotechnol Date: 2014-11-14 Impact factor: 8.429
Authors: Arpita Das; Ashok K Parihar; Deepa Saxena; Deepak Singh; K D Singha; K P S Kushwaha; Ramesh Chand; R S Bal; Subhash Chandra; Sanjeev Gupta Journal: Front Plant Sci Date: 2019-07-10 Impact factor: 5.753
Authors: Ashraf El-Kereamy; Yong-Mei Bi; Kosala Ranathunge; Perrin H Beatty; Allen G Good; Steven J Rothstein Journal: PLoS One Date: 2012-12-14 Impact factor: 3.240
Authors: Manus P M Thoen; Nelson H Davila Olivas; Karen J Kloth; Silvia Coolen; Ping-Ping Huang; Mark G M Aarts; Johanna A Bac-Molenaar; Jaap Bakker; Harro J Bouwmeester; Colette Broekgaarden; Johan Bucher; Jacqueline Busscher-Lange; Xi Cheng; Emilie F Fradin; Maarten A Jongsma; Magdalena M Julkowska; Joost J B Keurentjes; Wilco Ligterink; Corné M J Pieterse; Carolien Ruyter-Spira; Geert Smant; Christa Testerink; Björn Usadel; Joop J A van Loon; Johan A van Pelt; Casper C van Schaik; Saskia C M van Wees; Richard G F Visser; Roeland Voorrips; Ben Vosman; Dick Vreugdenhil; Sonja Warmerdam; Gerrie L Wiegers; Joost van Heerwaarden; Willem Kruijer; Fred A van Eeuwijk; Marcel Dicke Journal: New Phytol Date: 2016-10-04 Impact factor: 10.151