Clarisse Maria Barbosa Fonseca1, Tarsia Giabardo Silva Mendonça2, Cristian Francisco de Carvalho Pereira3, Gabriel Martins de Barros2, Andrezza Braga Soares da Silva4, Maria Michele Araújo de Sousa Cavalcante4, Maria do Socorro Pires E Cruz5, Airton Mendes Conde Júnior2. 1. Master Program in Science and Health, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, CEP: 64059-550, Teresina, Piauí, Brazil. Electronic address: clarissembfonseca@gmail.com. 2. Master Program in Science and Health, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, CEP: 64059-550, Teresina, Piauí, Brazil. 3. Department of Morphology, Health Sciences Center, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, CEP: 64059-550, Teresina, Piauí, Brazil. 4. Doctoral Program in Technologies Applied to Animals of Regional Interest, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, CEP: 64059-550, Teresina, Piauí, Brazil. 5. Department of Veterinary Morphophysiology, Center for Agricultural Sciences, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, CEP: 64059-550, Teresina, Piauí, Brazil.
Abstract
OBJECTIVE: The objective of this study was to perform a histopathological, morphometric and proteomic study of the parotid gland of dogs naturally infected with Leishmania infantum treated and not treated with Allopurinol. DESIGN: Parotid glands from 14 dogs were used, divided into two groups: untreated and treated with oral allopurinol (20 mg / kg, once daily for 90 days). After adequate dissection, the organs were submitted to histopathological, histomorphometric and immunohistochemical techniques, using the monoclonal anti-β-catenin antibody. RESULTS: Histopathological evaluation of treated and untreated groups showed acinar hypertrophy, structural disorganization of the nucleus and cytoplasm. There was an increase in the area and perimeter of the parotid acini in the experimental groups. The immunostaining of the β-catenin protein in the membrane was severely reduced in the treated and untreated groups. CONCLUSIONS: These findings suggest that Leishmania infantum infection and treatment with Allopurinol alter the tissue structure of the parotid gland in dogs, promoting an increase in the acinar volume and a decrease in the expression of β-catenin in cell membranes.
OBJECTIVE: The objective of this study was to perform a histopathological, morphometric and proteomic study of the parotid gland of dogs naturally infected with Leishmania infantum treated and not treated with Allopurinol. DESIGN: Parotid glands from 14 dogs were used, divided into two groups: untreated and treated with oral allopurinol (20 mg / kg, once daily for 90 days). After adequate dissection, the organs were submitted to histopathological, histomorphometric and immunohistochemical techniques, using the monoclonal anti-β-catenin antibody. RESULTS: Histopathological evaluation of treated and untreated groups showed acinar hypertrophy, structural disorganization of the nucleus and cytoplasm. There was an increase in the area and perimeter of the parotid acini in the experimental groups. The immunostaining of the β-catenin protein in the membrane was severely reduced in the treated and untreated groups. CONCLUSIONS: These findings suggest that Leishmania infantum infection and treatment with Allopurinol alter the tissue structure of the parotid gland in dogs, promoting an increase in the acinar volume and a decrease in the expression of β-catenin in cell membranes.