[In vivo and in vitro studies of a cytoplasmic polyhedrosis virus isolated from Euxoa scandens, Riley (Noctuidae, Agrotinae)(author's transl)].

A virus isolated from the larvaes of Euxoa scandens (Noctuidae, Agrotinae) was studied by histological and histochemical techniques, and by electron microscopic observation of virus suspensions and ultrathin sections of infected tissues. Our studies demonstrated that the virus in icosahedral in shape, measures 70 nm and belongs to the cytoplasmic polyhedrosis virus group. Only the midgut cells of the larvae were found to be infected and the virus particles were localized in inclusion bodies most of which had a paraspherical, polyhedral shape. It was possible to infect Lymantria dispar L. cells in vitro with free virus or with extracts obtained from the diseased larvae. The virogenic stroma and the first small inclusion bodies in this stroma were detected in the cytoplasm at 18 and 36 hours post infection, respectively. These polyhedra had a cubical shape, reached their maximum size after 48 hours and were located in the cytoplasm of over 90 percent of the cells.