Rajanbir Kaur1, Arpna Kumari1, Gauri Sharma1, Drishtant Singh2, Rajinder Kaur1. 1. Department of Botanical and Environmental Sciences, Amritsar, Punjab, 143005, India. 2. Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, Punjab, 143005, India.
Abstract
AIM: The objectives of the present study was to explore the benzyl butyl phthalate (BBP) and dimethyl phthalate (DMP) degradation potential of Bacillus marisflavi RR014 isolated from the tap water of public toilet and also to optimize the phthalates degradation process using response surface methodology. METHODS AND RESULTS: The minimal salt medium was used for the biodegradation analysis of phthalates. The quantification of phthalates and their intermediate metabolites identification were done by using UHPLC and LC-MS/MS respectively. The results revealed that Bacillus marisflavi RR014 is capable of degrading both the phthalates under varying pH, temperature and salinity conditions. The formation of phthalic acid from the breakdown of BBP and DMP (500 mg/L) in the medium was observed after 24 h. After 72 h, 61% of BBP and 98.9% of DMP in the medium was degraded as monitored by UHPLC. The identification of intermediate metabolites by LC-MS/MS revealed that hydrolysis of BBP and DMP produces phthalic acid. CONCLUSIONS: The degradation rate of both the phthalates was increased as the parameters increased up to an optimum level. The three environmental factors (pH, temperature and salt concentration) strongly affect the rate of degradation of both the phthalates. The maximum degradation rate for both the phthalates was achieved at pH 7, temperature 35ºC and salt concentration of 1% as observed from the central composite experimental design. SIGNIFICANCE AND IMPACT OF STUDY: It is the first report on the phthalates biodegradation potential of Bacillus marisflavi RR014 isolated from the tap water of public toilet. The bacterium is capable of degrading benzyl butyl phthalate and di-methyl phthalate under varying pH, temperature and salinity, therefore, ideal to treat the phthalate contaminated environments. This article is protected by copyright. All rights reserved.
AIM: The objectives of the present study was to explore the benzyl butyl phthalate (BBP) and dimethyl phthalate (DMP) degradation potential of Bacillus marisflavi RR014 isolated from the tap water of public toilet and also to optimize the phthalates degradation process using response surface methodology. METHODS AND RESULTS: The minimal salt medium was used for the biodegradation analysis of phthalates. The quantification of phthalates and their intermediate metabolites identification were done by using UHPLC and LC-MS/MS respectively. The results revealed that Bacillus marisflavi RR014 is capable of degrading both the phthalates under varying pH, temperature and salinity conditions. The formation of phthalic acid from the breakdown of BBP and DMP (500 mg/L) in the medium was observed after 24 h. After 72 h, 61% of BBP and 98.9% of DMP in the medium was degraded as monitored by UHPLC. The identification of intermediate metabolites by LC-MS/MS revealed that hydrolysis of BBP and DMP produces phthalic acid. CONCLUSIONS: The degradation rate of both the phthalates was increased as the parameters increased up to an optimum level. The three environmental factors (pH, temperature and salt concentration) strongly affect the rate of degradation of both the phthalates. The maximum degradation rate for both the phthalates was achieved at pH 7, temperature 35ºC and salt concentration of 1% as observed from the central composite experimental design. SIGNIFICANCE AND IMPACT OF STUDY: It is the first report on the phthalates biodegradation potential of Bacillus marisflavi RR014 isolated from the tap water of public toilet. The bacterium is capable of degrading benzyl butyl phthalate and di-methyl phthalate under varying pH, temperature and salinity, therefore, ideal to treat the phthalate contaminated environments. This article is protected by copyright. All rights reserved.