Reinhard Dolp1,2,3, Gertraud Eylert1,3,4, Christopher Auger1, Ayesha Aijaz1, Yufei Andy Chen1, Saeid Amini-Nik1,5,6, Alexandra Parousis1, Andrea-Kaye Datu1, Marc G Jeschke7,8,9,10. 1. Sunnybrook Research Institute, Toronto, Canada. 2. Department of Psychiatry, Queen's University, Kingston, Canada. 3. Institute of Medical Science, University of Toronto, Ontario, Canada. 4. Division of Plastic, Aesthetic and Reconstructive Surgery, Medical University of Graz, Graz, Austria. 5. Department of Laboratory Medicine and Pathobiology (LMP), University of Toronto, Toronto, Canada. 6. SGS Harrison Research Laboratories, SGS North America, New York Metropolitan Area, Union, NJ, USA. 7. Sunnybrook Research Institute, Toronto, Canada. Marc.Jeschke@sunnybrook.ca. 8. Department of Immunology, Ross Tilley Burn Centre, Sunnybrook Health Sciences Centre, 2075 Bayview Ave., Toronto, ON, M4N 3M5, Canada. Marc.Jeschke@sunnybrook.ca. 9. Division of Plastic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine, University of Toronto, Toronto, Canada. Marc.Jeschke@sunnybrook.ca. 10. Ross Tilley Burn Centre, Sunnybrook Health Science Centre, Toronto, Canada. Marc.Jeschke@sunnybrook.ca.
Abstract
INTRODUCTION: Burned human skin, which is routinely excised and discarded, contains viable mesenchymal stromal/stem cells (burn-derived mesenchymal stromal/stem cells; BD-MSCs). These cells show promising potential to enable and aid wound regeneration. However, little is known about their cell characteristics and biological function. OBJECTIVES: This study had two aims: first, to assess critical and cellular characteristics of BD-MSCs and, second, to compare those results with multipotent well-characterized MSCs from Wharton's jelly of human umbilical cords (umbilical cord mesenchymal stromal/stem cells, UC-MSCs). METHODS: BD- and UC-MSCs were compared using immunophenotyping, multi-lineage differentiation, seahorse analysis for glycolytic and mitochondrial function, immune surface markers, and cell secretion profile assays. RESULTS: When compared to UC-MSCs, BD-MSCs demonstrated a lower mesenchymal differentiation capacity and altered inflammatory cytokine secretomes at baseline and after stimulation with lipopolysaccharides. No significant differences were found in population doubling time, colony formation, cell proliferation cell cycle, production of reactive oxygen species, glycolytic and mitochondrial function, and in the expression of major histocompatibility complex I and II and toll-like receptor (TLR). IMPORTANCE, TRANSLATION: This study reveals valuable insights about MSCs obtained from burned skin and show comparable cellular characteristics with UC-MSCs, highlighting their potentials in cell therapy and skin regeneration.
INTRODUCTION: Burned human skin, which is routinely excised and discarded, contains viable mesenchymal stromal/stem cells (burn-derived mesenchymal stromal/stem cells; BD-MSCs). These cells show promising potential to enable and aid wound regeneration. However, little is known about their cell characteristics and biological function. OBJECTIVES: This study had two aims: first, to assess critical and cellular characteristics of BD-MSCs and, second, to compare those results with multipotent well-characterized MSCs from Wharton's jelly of human umbilical cords (umbilical cord mesenchymal stromal/stem cells, UC-MSCs). METHODS: BD- and UC-MSCs were compared using immunophenotyping, multi-lineage differentiation, seahorse analysis for glycolytic and mitochondrial function, immune surface markers, and cell secretion profile assays. RESULTS: When compared to UC-MSCs, BD-MSCs demonstrated a lower mesenchymal differentiation capacity and altered inflammatory cytokine secretomes at baseline and after stimulation with lipopolysaccharides. No significant differences were found in population doubling time, colony formation, cell proliferation cell cycle, production of reactive oxygen species, glycolytic and mitochondrial function, and in the expression of major histocompatibility complex I and II and toll-like receptor (TLR). IMPORTANCE, TRANSLATION: This study reveals valuable insights about MSCs obtained from burned skin and show comparable cellular characteristics with UC-MSCs, highlighting their potentials in cell therapy and skin regeneration.
Authors: Andrew M Tager; Richard L Kradin; Peter LaCamera; Scott D Bercury; Gabriele S V Campanella; Carol P Leary; Vasiliy Polosukhin; Long-Hai Zhao; Hideo Sakamoto; Timothy S Blackwell; Andrew D Luster Journal: Am J Respir Cell Mol Biol Date: 2004-06-17 Impact factor: 6.914
Authors: Beate M Lichtenberger; Esther Hoste; Kai Kretzschmar; Ryan R Driskell; Ben D Simons; Marika Charalambous; Sacri R Ferron; Yann Herault; Guillaume Pavlovic; Anne C Ferguson-Smith; Fiona M Watt Journal: Nature Date: 2013-12-12 Impact factor: 49.962