DNA methylation levels in acute human leukemia.

We have studied the overall 5-methylcytosine content and the percentage of methylated CpG-dinucleotides in 25 cases of untreated human acute leukemias. For comparison, normal leucocyte subpopulations were similarly analyzed. The methylation levels in normal white blood cell DNA varied in the same range as those in leukemia cells with no apparent hypomethylation in tumor cell DNA. Such hypomethylation, however, was found in a patient studied in first and second relapses of the disease. These data suggest that genome-wide demethylation, a characteristic of other tumors, does not accompany leukemic transformation.