Literature DB >> 33593833

Identification and characterization of two novel ISCR1-associated genes dfrA42 and dfrA43 encoding trimethoprim resistant dihydrofolate reductases.

Ling Li1, Mengge Zhang1, Wenjia Wang1, Ruirui Xia1, Yanan Ma1, Xin Wei1, Xinhua Wang2, Xiaomin Sun3, Xiaohong Xie4, Shiling Xie4, Mingyu Wang5, Hai Xu5.   

Abstract

Two novel ISCR1-associated dfr genes, dfrA42 and dfrA43, were identified from trimethoprim (TMP)-resistant Proteus strains and were shown to confer high level TMP resistance (MIC ≥ 1024 mg/L) when cloned into Escherichia coli These genes were hosted by complex class 1 integrons suggesting their potentials for dissemination. Analysis of enzymatic parameters and TMP affinity were performed, suggesting that the mechanism of TMP resistance for these novel DHFRs is the reduction of binding with TMP.
Copyright © 2021 American Society for Microbiology.

Entities:  

Year:  2021        PMID： 33593833      PMCID： PMC8092886          DOI： 10.1128/AAC.02010-20

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  19 in total

1.  Integrons and gene cassettes in the enterobacteriaceae.

Authors:  P A White; C J McIver; W D Rawlinson
Journal:  Antimicrob Agents Chemother       Date:  2001-09       Impact factor: 5.191

2.  Class 1 and class 2 integrons in multidrug-resistant gram-negative bacteria isolated from the Salmon River, British Columbia.

Authors:  Hai Xu; Klaas Broersma; Vivian Miao; Julian Davies
Journal:  Can J Microbiol       Date:  2011-05-31       Impact factor: 2.419

3.  A novel trimethoprim resistance gene, dfrA38, found in a sporadic Acinetobacter baumannii isolate.

Authors:  Stephanie J Ambrose; Ruth M Hall
Journal:  J Antimicrob Chemother       Date:  2020-12-01       Impact factor: 5.790

Review 4.  Trimethoprim and sulfonamide resistance.

Authors:  P Huovinen; L Sundström; G Swedberg; O Sköld
Journal:  Antimicrob Agents Chemother       Date:  1995-02       Impact factor: 5.191

Review 5.  Gene cassettes: a new class of mobile element.

Authors:  G D Recchia; R M Hall
Journal:  Microbiology       Date:  1995-12       Impact factor: 2.777

6.  The 2017 Update of the German Clinical Guideline on Epidemiology, Diagnostics, Therapy, Prevention, and Management of Uncomplicated Urinary Tract Infections in Adult Patients. Part II: Therapy and Prevention.

Authors:  Jennifer Kranz; Stefanie Schmidt; Cordula Lebert; Laila Schneidewind; Falitsa Mandraka; Mirjam Kunze; Sina Helbig; Winfried Vahlensieck; Kurt Naber; Guido Schmiemann; Florian M Wagenlehner
Journal:  Urol Int       Date:  2018-03-14       Impact factor: 2.089

7.  Trimethoprim resistance determined by R factors.

Authors:  M P Fleming; N Datta; R N Grüneberg
Journal:  Br Med J       Date:  1972-03-18

8.  Identification of plasmid-mediated quinolone resistance qnr genes in multidrug-resistant Gram-negative bacteria from hospital wastewaters and receiving waters in the Jinan area, China.

Authors:  Ruirui Xia; Ye Ren; Hai Xu
Journal:  Microb Drug Resist       Date:  2013-07-11       Impact factor: 3.431

9.  Characterization of trimethoprim resistant E. coli dihydrofolate reductase mutants by mass spectrometry and inhibition by propargyl-linked antifolates.

Authors:  Michael Cammarata; Ross Thyer; Michael Lombardo; Amy Anderson; Dennis Wright; Andrew Ellington; Jennifer S Brodbelt
Journal:  Chem Sci       Date:  2017-03-28       Impact factor: 9.825

10.  Exploration into the origins and mobilization of di-hydrofolate reductase genes and the emergence of clinical resistance to trimethoprim.

Authors:  Miquel Sánchez-Osuna; Pilar Cortés; Montserrat Llagostera; Jordi Barbé; Ivan Erill
Journal:  Microb Genom       Date:  2020-11
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