| Literature DB >> 33590351 |
Mukhit B Orynbayev1,2, Raikhan K Nissanova3,4, Berik M Khairullin3, Arman Issimov3,5, Kunsulu D Zakarya3, Kulyaisan T Sultankulova3, Lespek B Kutumbetov3, Ali B Tulendibayev3, Balzhan Sh Myrzakhmetova3, Erbol D Burashev3, Sergazy S Nurabayev3, Olga V Chervyakova3, Aziz K Nakhanov3, Richard A Kock6.
Abstract
This study describes the registration of the first cases of lumpy skin disease in July 2016 in the Republic of Kazakhstan. In the rural district of Makash, Kurmangazinsky district of Atyrau region, 459 cattle fell ill and 34 died (morbidity 12.9% and mortality 0.96%). To determine the cause of the disease, samples were taken from sick and dead animals, as well as from insects and ticks. LSDV DNA was detected by PCR in all samples from dead animals and ticks (Dermacentor marginatus and Hyalomma asiaticum), in 14.29% of samples from horseflies (Tabanus bromius), and in one of the samples from two Stomoxys calcitrans flies. The reproductive LSD virus was isolated from organs of dead cattle and insects in the culture of LT and MDBK cells. The virus accumulated in cell cultures of LT and MDBK at the level of the third passage with titers in the range of 5.5-5.75 log 10 TCID50/cm3. Sequencing of the GPCR gene allowed us to identify this virus as a lumpy skin disease virus.Entities:
Keywords: Insect; Kazakhstan; Lumpy skin disease; Tick; Vector-borne disease
Mesh：
Year: 2021 PMID： 33590351 PMCID： PMC7884366 DOI： 10.1007/s11250-021-02613-6
Source DB: PubMed Journal: Trop Anim Health Prod ISSN： 0049-4747 Impact factor: 1.559