Peng Shen1, Lili Qu1, Jingjing Wang1, Quchen Ding1, Chuanwen Zhou2, Rui Xie2, Honggang Wang2, Guozhong Ji3. 1. The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210000, China. 2. The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an, 223300, China. 3. The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210000, China. jgz@njmu.edu.cn.
Abstract
BACKGROUND: Long intergenic non-protein coding RNA 00342 (LINC00342) has been identified as a novel oncogene. However, the functional role of LINC00342 in colorectal cancer (CRC) remains unclear. METHODS: The expression of LINC00342 is detected by real-time PCR (RT-PCR) analysis. Cell proliferation, migration and invasion and xenograft model are examined to analyze the biological functions of LINC00342 in vitro and in vivo using colony formation, would healing and transwell analyses. Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays are used to identify the target interactions between LINC00342, miR-19a-3p and aminopeptidase like 1 (NPEPL1). RESULTS: LINC00342 was highly expressed in CRC. Down-regulation of LINC00342 inhibited cell proliferation and metastasis of CRC cells. Moreover, knocking down LINC00342 inhibited the tumor growth in vivo. Mechanistic investigation revealed that LINC00342 might sponge miR-19a-3p to regulate NPEPL1 expression. Further investigation indicated that the ontogenesis facilitated by LINC00342 was inhibited due to the depletion of NPEPL1. CONCLUSION: LINC00342 promotes CRC progression by competitively binding miR-19a-3p with NPEPL1.
BACKGROUND: Long intergenic non-protein coding RNA 00342 (LINC00342) has been identified as a novel oncogene. However, the functional role of LINC00342 in colorectal cancer (CRC) remains unclear. METHODS: The expression of LINC00342 is detected by real-time PCR (RT-PCR) analysis. Cell proliferation, migration and invasion and xenograft model are examined to analyze the biological functions of LINC00342 in vitro and in vivo using colony formation, would healing and transwell analyses. Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays are used to identify the target interactions between LINC00342, miR-19a-3p and aminopeptidase like 1 (NPEPL1). RESULTS:LINC00342 was highly expressed in CRC. Down-regulation of LINC00342 inhibited cell proliferation and metastasis of CRC cells. Moreover, knocking down LINC00342 inhibited the tumor growth in vivo. Mechanistic investigation revealed that LINC00342 might sponge miR-19a-3p to regulate NPEPL1 expression. Further investigation indicated that the ontogenesis facilitated by LINC00342 was inhibited due to the depletion of NPEPL1. CONCLUSION:LINC00342 promotes CRC progression by competitively binding miR-19a-3p with NPEPL1.
Authors: Wen Deng; Gongxian Wang; Huanhuan Deng; Yan Yan; Ke Zhu; Ru Chen; Xiaoqiang Liu; Luyao Chen; Tao Zeng; Bin Fu Journal: Int J Gen Med Date: 2021-12-14