Yingxue Xue1, Yifei Yin1, He Li2, Mingyu Chi1, Jiaxin Guo1, Guihua Cui1, Wenliang Li1,3. 1. School of Pharmacy, Jilin Medical University, Jilin, China. 2. Research and Development Department, NCPC Hebei Lexin Pharmaceutical Co., Ltd., Hebei, China. 3. Jilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical University, Jilin, China.
Abstract
A dimeric Keggin-type phosphotungstate (ODA)10[(PW11FeO39)2O]·9H2O (abbreviated as ODA10[(PW11Fe)2], ODA = octadecyltrimethylammonium bromide) was synthesized and investigated comprehensively its antitumor activity on MCF-7 and A549 cells. The dimeric structure and amorphous morphology were characterized by FT-IR, UV-vis-DRS, SEM and XRD. The in vitro MTT assay of ODA10[(PW11Fe)2] showed anticancer activity on MCF-7 and A549 cells in a dose- and time-dependent manner, and the IC50 values for MCF-7 and A549 cells at 48 h were 5.83 μg/ml and 3.23 μg/ml, respectively. The images of the ODA10[(PW11Fe)2]-treated cells observed by inverted biological microscope exhibited the characteristic morphology of apoptosis. Flow cytometric analysis showed cell apoptosis and cycle arrested at S phase induced by ODA10[(PW11Fe)2]. The above results illuminated the main mechanism of the antitumor action of ODA10[(PW11Fe)2] on MCF-7 and A549 cells, indicating that this dimeric phosphotungstate is a promising anticancer drug.
A dimeric Keggin-type phosphotungstaten>an class="Chemical">(ODA)10[(PW11FeO39)2O]·9H2O (abbreviated as ODA10[(PW11Fe)2], ODA = octadecyltrimethylammonium bromide) was synthesized and investigated comprehensively its antitumor activity on MCF-7 and A549 cells. The dimeric structure and amorphous morphology were characterized by FT-IR, UV-vis-DRS, SEM and XRD. The in vitro MTT assay of ODA10[(PW11Fe)2] showed anticancer activity on MCF-7 and A549 cells in a dose- and time-dependent manner, and the IC50 values for MCF-7 and A549 cells at 48 h were 5.83 μg/ml and 3.23 μg/ml, respectively. The images of the ODA10[(PW11Fe)2]-treated cells observed by inverted biological microscope exhibited the characteristic morphology of apoptosis. Flow cytometric analysis showed cell apoptosis and cycle arrested at S phase induced by ODA10[(PW11Fe)2]. The above results illuminated the main mechanism of the antitumor action of ODA10[(PW11Fe)2] on MCF-7 and A549 cells, indicating that this dimeric phosphotungstate is a promising anticancer drug.
Authors: D A Judd; J H Nettles; N Nevins; J P Snyder; D C Liotta; J Tang; J Ermolieff; R F Schinazi; C L Hill Journal: J Am Chem Soc Date: 2001-02-07 Impact factor: 15.419
Authors: Christa E Müller; Jamshed Iqbal; Younis Baqi; Herbert Zimmermann; Anita Röllich; Holger Stephan Journal: Bioorg Med Chem Lett Date: 2006-09-25 Impact factor: 2.823