Rocío Guizar-Heredia1, Armando R Tovar2, Omar Granados-Portillo2, Edgar Pichardo-Ontiveros2, Adriana Flores-López2, Luis E González-Salazar2, Liliana Arteaga-Sanchez2, Isabel Medina-Vera3, Ximena Orozco-Ruiz2, Nimbe Torres2, Lilia G Noriega4, Martha Guevara-Cruz5. 1. Departamento de Fisiología de Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán, México D.F., Mexico. Electronic address: rg_heredia@hotmail.com. 2. Departamento de Fisiología de Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán, México D.F., Mexico. 3. Departamento de Metodología de la Investigación, Instituto Nacional de Pediatría, Ciudad de México, Mexico. 4. Departamento de Fisiología de Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán, México D.F., Mexico. Electronic address: lgnoriegal@gmail.com. 5. Departamento de Fisiología de Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán, México D.F., Mexico. Electronic address: marthaguevara8@yahoo.com.mx.
Abstract
BACKGROUND & AIMS: The amino acid profile of young adults is modified by sex, body mass index (BMI) and insulin resistance (IR). However, we do not know if age or the presence of specific polymorphisms in the genes of BCAT2 and BCKDH contribute to changes in the amino acid profile, especially in subjects with obesity. Therefore, we have evaluated the effect of age, the presence of IR and the polymorphisms of BCAT2 rs11548193 and BCKDH rs45500792 on the concentration of amino acids in subjects with obesity. METHODS: This was a cross-sectional study conducted with 487 subjects with obesity. Participants underwent a physical examination in which their clinical history was obtained and a blood sample was taken for biochemical, hormonal, and DNA analysis. RESULTS: Adults <30 years old with obesity had higher levels of alanine, arginine, aspartate, histidine, leucine, lysine, methionine, phenylalanine, proline, serine and valine than adults ≥30 years old. Interestingly, regardless of age, we found that arginine, aspartate, serine decreased, while proline and tyrosine increased in the presence of IR; tyrosine and sum of branched-chain amino acids (∑BCAA) were the amino acids that increased in the presence of BCAT2 rs11548193 and BCKDH rs45500792 polymorphisms. CONCLUSIONS: We found that the amino acid profiles of subjects with obesity are differentially modified by age, the presence of IR, and the presence of the BCAT2 rs11548193 and BCKDH rs45500792 polymorphisms.
BACKGROUND & AIMS: The amino acid profile of young adults is modified by sex, body mass index (BMI) and insulin resistance (IR). However, we do not know if age or the presence of specific polymorphisms in the genes of BCAT2 and BCKDH contribute to changes in the amino acid profile, especially in subjects with obesity. Therefore, we have evaluated the effect of age, the presence of IR and the polymorphisms of BCAT2 rs11548193 and BCKDH rs45500792 on the concentration of amino acids in subjects with obesity. METHODS: This was a cross-sectional study conducted with 487 subjects with obesity. Participants underwent a physical examination in which their clinical history was obtained and a blood sample was taken for biochemical, hormonal, and DNA analysis. RESULTS: Adults <30 years old with obesity had higher levels of alanine, arginine, aspartate, histidine, leucine, lysine, methionine, phenylalanine, proline, serine and valine than adults ≥30 years old. Interestingly, regardless of age, we found that arginine, aspartate, serine decreased, while proline and tyrosine increased in the presence of IR; tyrosine and sum of branched-chain amino acids (∑BCAA) were the amino acids that increased in the presence of BCAT2 rs11548193 and BCKDH rs45500792 polymorphisms. CONCLUSIONS: We found that the amino acid profiles of subjects with obesity are differentially modified by age, the presence of IR, and the presence of the BCAT2 rs11548193 and BCKDH rs45500792 polymorphisms.