Literature DB >> 33579792

The effect of the D614G substitution on the structure of the spike glycoprotein of SARS-CoV-2.

Donald J Benton1, Antoni G Wrobel1, Chloë Roustan2, Annabel Borg2, Pengqi Xu3,4, Stephen R Martin4, Peter B Rosenthal5, John J Skehel1, Steven J Gamblin1.   

Abstract

The majority of currently circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses have mutant spike glycoproteins that contain the D614G substitution. Several studies have suggested that spikes with this substitution are associated with higher virus infectivity. We use cryo-electron microscopy to compare G614 and D614 spikes and show that the G614 mutant spike adopts a range of more open conformations that may facilitate binding to the SARS-CoV-2 receptor, ACE2, and the subsequent structural rearrangements required for viral membrane fusion.
Copyright © 2021 the Author(s). Published by PNAS.

Entities:  

Keywords:  Coronavirus; D614G; SARS-CoV-2; cryo-EM; spike

Mesh：

Substances：

Year:  2021        PMID： 33579792      PMCID： PMC7936381          DOI： 10.1073/pnas.2022586118

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   12.779


  27 in total

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Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-01-22

5.  Evaluating the Effects of SARS-CoV-2 Spike Mutation D614G on Transmissibility and Pathogenicity.

Authors:  Erik Volz; Verity Hill; John T McCrone; Anna Price; David Jorgensen; Áine O'Toole; Joel Southgate; Robert Johnson; Ben Jackson; Fabricia F Nascimento; Sara M Rey; Samuel M Nicholls; Rachel M Colquhoun; Ana da Silva Filipe; James Shepherd; David J Pascall; Rajiv Shah; Natasha Jesudason; Kathy Li; Ruth Jarrett; Nicole Pacchiarini; Matthew Bull; Lily Geidelberg; Igor Siveroni; Ian Goodfellow; Nicholas J Loman; Oliver G Pybus; David L Robertson; Emma C Thomson; Andrew Rambaut; Thomas R Connor
Journal:  Cell       Date:  2020-11-19       Impact factor: 41.582

6.  SARS-CoV-2 and bat RaTG13 spike glycoprotein structures inform on virus evolution and furin-cleavage effects.

Authors:  Antoni G Wrobel; Donald J Benton; Pengqi Xu; Chloë Roustan; Stephen R Martin; Peter B Rosenthal; John J Skehel; Steven J Gamblin
Journal:  Nat Struct Mol Biol       Date:  2020-07-09       Impact factor: 15.369

7.  SARS-CoV-2 spike-protein D614G mutation increases virion spike density and infectivity.

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Authors:  Christine Toelzer; Kapil Gupta; Sathish K N Yadav; Ufuk Borucu; Andrew D Davidson; Maia Kavanagh Williamson; Deborah K Shoemark; Frederic Garzoni; Oskar Staufer; Rachel Milligan; Julien Capin; Adrian J Mulholland; Joachim Spatz; Daniel Fitzgerald; Imre Berger; Christiane Schaffitzel
Journal:  Science       Date:  2020-09-21       Impact factor: 47.728

9.  Tracking Changes in SARS-CoV-2 Spike: Evidence that D614G Increases Infectivity of the COVID-19 Virus.

Authors:  Bette Korber; Will M Fischer; Sandrasegaram Gnanakaran; Hyejin Yoon; James Theiler; Werner Abfalterer; Nick Hengartner; Elena E Giorgi; Tanmoy Bhattacharya; Brian Foley; Kathryn M Hastie; Matthew D Parker; David G Partridge; Cariad M Evans; Timothy M Freeman; Thushan I de Silva; Charlene McDanal; Lautaro G Perez; Haili Tang; Alex Moon-Walker; Sean P Whelan; Celia C LaBranche; Erica O Saphire; David C Montefiori
Journal:  Cell       Date:  2020-07-03       Impact factor: 66.850

10.  SARS-CoV-2 viral spike G614 mutation exhibits higher case fatality rate.

Authors:  Manuel Becerra-Flores; Timothy Cardozo
Journal:  Int J Clin Pract       Date:  2020-06-03       Impact factor: 3.149
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2.  Engineered ACE2-Fc counters murine lethal SARS-CoV-2 infection through direct neutralization and Fc-effector activities.

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4.  An antigen microarray protocol for COVID-19 serological analysis.

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8.  Altered Local Interactions and Long-Range Communications in UK Variant (B.1.1.7) Spike Glycoprotein.

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9.  Landscape-Based Mutational Sensitivity Cartography and Network Community Analysis of the SARS-CoV-2 Spike Protein Structures: Quantifying Functional Effects of the Circulating D614G Variant.

Authors:  Gennady M Verkhivker; Steve Agajanian; Deniz Yasar Oztas; Grace Gupta
Journal:  ACS Omega       Date:  2021-06-09

Review 10.  SARS-CoV-2 one year on: evidence for ongoing viral adaptation.

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