Junming Luo1,2, Xiaoqin Luo2, Zhili Duan2, Wenbin Bai3, Xiaoming Che3, Zhongshu Shan3, Xiaona Li2, Jinwu Peng4,5,6. 1. Department of Pathology, Xiangya Changde Hospital, Changde, 415000, Hunan, China. 2. Department of Pathology, Qinghai Provincial People's Hospital, Xining, 810007, Qinghai, China. 3. Department of Orthopedic Surgery, Qinghai Provincial People's Hospital, Xining, 810007, Qinghai, China. 4. Department of Pathology, Xiangya Changde Hospital, Changde, 415000, Hunan, China. jinwupeng2005@aliyun.com. 5. Department of Pathology, Xiangya Basic Medical School, Central South University, Changsha, 410013, Hunan, China. jinwupeng2005@aliyun.com. 6. Department of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China. jinwupeng2005@aliyun.com.
Abstract
BACKGROUND: Osteoarthritis (OA) is thought to be the most prevalent chronic joint disease, especially in Tibet of China. Here, we aimed to explore the integrative lncRNA and mRNA landscape between the OA patients of Tibet and Han. METHODS: The lncRNA and mRNA expression microarray profiling was performed by SurePrint G3 Human Gene Expression 8x60K v2 Microarray in articular cartilage samples from OA patients of Han nationality and Tibetans, followed by GO, KEGG, and trans-regulation and cis-regulation analysis of lncRNA and mRNA. RESULTS: We found a total of 117 lncRNAs and 297 mRNAs differently expressed in the cartilage tissues of Tibetans (n = 5) comparing with those of Chinese Han (n = 3), in which 49 lncRNAs and 158 mRNAs were upregulated, and 68 lncRNAs and 139 mRNAs were downregulated. GO and KEGG analysis showed that several unreported biological processes and signaling pathways were particularly identified. LncRNA-mRNA co-expression analysis revealed a remarkable lncRNA-mRNA relationship, in which OTOA may play a critical role in the different mechanisms of the OA progression between Tibetans and Chinese Han. CONCLUSION: This study identified different lncRNA/mRNA expression profiling between OA patients of Tibetans and Han, which were involved in many characteristic biological processes and signaling pathways.
BACKGROUND:Osteoarthritis (OA) is thought to be the most prevalent chronic joint disease, especially in Tibet of China. Here, we aimed to explore the integrative lncRNA and mRNA landscape between the OA patients of Tibet and Han. METHODS: The lncRNA and mRNA expression microarray profiling was performed by SurePrint G3 Human Gene Expression 8x60K v2 Microarray in articular cartilage samples from OA patients of Han nationality and Tibetans, followed by GO, KEGG, and trans-regulation and cis-regulation analysis of lncRNA and mRNA. RESULTS: We found a total of 117 lncRNAs and 297 mRNAs differently expressed in the cartilage tissues of Tibetans (n = 5) comparing with those of Chinese Han (n = 3), in which 49 lncRNAs and 158 mRNAs were upregulated, and 68 lncRNAs and 139 mRNAs were downregulated. GO and KEGG analysis showed that several unreported biological processes and signaling pathways were particularly identified. LncRNA-mRNA co-expression analysis revealed a remarkable lncRNA-mRNA relationship, in which OTOA may play a critical role in the different mechanisms of the OA progression between Tibetans and Chinese Han. CONCLUSION: This study identified different lncRNA/mRNA expression profiling between OA patients of Tibetans and Han, which were involved in many characteristic biological processes and signaling pathways.
Authors: R Moreno-Reyes; C Suetens; F Mathieu; F Begaux; D Zhu; M T Rivera; M Boelaert; J Nève; N Perlmutter; J Vanderpas Journal: N Engl J Med Date: 1998-10-15 Impact factor: 91.245