Holistic insights into extracellular polymeric substance (EPS) in anammosx bacterial matrix and the potential sustainable biopolymer recovery: A review.

Anaerobic ammonia oxidation (anammox) process has been proven to be a favorable and innovative process, for treatment of nitrogen-rich wastewater due to decreased oxygen and carbon requirements at very high nitrogen loading rates. Anammox process is mainly operated through biofilm or granular sludge structures, as for such slow-growing microorganisms, elevated settling velocity of granules allows for adequate biomass retention and lowered potential risk of washouts. Stability of granular sludge biomass is extremely critical, yet the formation mechanism is poorly understood. There are number of important functions linked to Extracellular Polymeric Substance (EPS) in anammox bacterial matrix, such as; structural stability, aggregation promotion, maintenance of physical structure in the granules, water preserving and protective cell barrier. There is an increasing demand to introduce accurate methods for proper EPS extraction and characterization, to expand the perception of anammox granule stability and potential resource recovery. Analyzing EPS with a focus on various (mechanical and physical) properties can lead to biopolymer production from granular sludge. Biopolymers such as EPS are attractive alternatives substituting the conventional chemical polymers furthermore their recovery from the waste sludge and the potential applications in industrial sectors, leads to a radical enhancement of both environmental and economical sustainability, accelerating the circular economy advancements. Here, this study aims to overview the newest understanding on the structure of anammox sludge EPS, obtained recently and to assess the potential challenges and prospects to identify the knowledge gaps towards constructing an inclusive anammox EPS recovery and characterization procedure.