Literature DB >> 33574331

Chromosome-scale genome assembly of the sea louse Caligus rogercresseyi by SMRT sequencing and Hi-C analysis.

Cristian Gallardo-Escárate1,2, Valentina Valenzuela-Muñoz3,4, Gustavo Nuñez-Acuña3,4, Diego Valenzuela-Miranda3,4, Ana Teresa Gonçalves3,4, Hugo Escobar-Sepulveda3,4, Ivan Liachko5, Bradley Nelson5, Steven Roberts6, Wesley Warren7.   

Abstract

Caligus rogercresseyi, commonly known as sea louse, is an ectoparasite copepod that impacts the salmon aquaculture in Chile, causing losses of hundreds of million dollars per year. In this study, we report a chromosome-scale assembly of the sea louse (C. rogercresseyi) genome based on single-molecule real-time sequencing (SMRT) and proximity ligation (Hi-C) analysis. Coding RNAs and non-coding RNAs, and specifically long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) were identified through whole transcriptome sequencing from different life stages. A total of 23,686 protein-coding genes and 12,558 non-coding RNAs were annotated. In addition, 6,308 lncRNAs and 5,774 miRNAs were found to be transcriptionally active from larvae to adult stages. Taken together, this genomic resource for C. rogercresseyi represents a valuable tool to develop sustainable control strategies in the salmon aquaculture industry.

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Year:  2021        PMID: 33574331      PMCID: PMC7878743          DOI: 10.1038/s41597-021-00842-w

Source DB:  PubMed          Journal:  Sci Data        ISSN: 2052-4463            Impact factor:   6.444


  41 in total

1.  MaskerAid: a performance enhancement to RepeatMasker.

Authors:  J A Bedell; I Korf; W Gish
Journal:  Bioinformatics       Date:  2000-11       Impact factor: 6.937

2.  Modulation of Atlantic salmon miRNome response to sea louse infestation.

Authors:  Valentina Valenzuela-Muñoz; Beatriz Novoa; Antonio Figueras; Cristian Gallardo-Escárate
Journal:  Dev Comp Immunol       Date:  2017-07-12       Impact factor: 3.636

3.  Transcriptome survey of the lipid metabolic pathways involved in energy production and ecdysteroid synthesis in the salmon louse Caligus rogercresseyi (Crustacea: Copepoda).

Authors:  Ana Teresa Gonçalves; Rodolfo Farlora; Cristian Gallardo-Escárate
Journal:  Comp Biochem Physiol B Biochem Mol Biol       Date:  2014-07-23       Impact factor: 2.231

4.  Repbase Update, a database of repetitive elements in eukaryotic genomes.

Authors:  Weidong Bao; Kenji K Kojima; Oleksiy Kohany
Journal:  Mob DNA       Date:  2015-06-02

5.  Discovery and annotation of long noncoding RNAs.

Authors:  John S Mattick; John L Rinn
Journal:  Nat Struct Mol Biol       Date:  2015-01       Impact factor: 15.369

6.  MicroRNA biogenesis pathway from the salmon louse (Caligus rogercresseyi): emerging role in delousing drug response.

Authors:  Diego Valenzuela-Miranda; Gustavo Nuñez-Acuña; Valentina Valenzuela-Muñoz; Sassan Asgari; Cristian Gallardo-Escárate
Journal:  Gene       Date:  2014-11-07       Impact factor: 3.688

7.  Chemoreception in the salmon louse Lepeophtheirus salmonis: an electrophysiology approach.

Authors:  D M Fields; M J Weissburg; H I Browman
Journal:  Dis Aquat Organ       Date:  2007-12-13       Impact factor: 1.802

Review 8.  How sea lice from salmon farms may cause wild salmonid declines in Europe and North America and be a threat to fishes elsewhere.

Authors:  Mark J Costello
Journal:  Proc Biol Sci       Date:  2009-07-08       Impact factor: 5.349

9.  Caligus rogercresseyi infestation is associated with Piscirickettsia salmonis-attributed mortalities in farmed salmonids in Chile.

Authors:  Gabriel Arriagada; Christopher Hamilton-West; Omid Nekouei; Claudia Foerster; Andrea Müller; Marcela Lara; Cristian Gallardo-Escárate
Journal:  Prev Vet Med       Date:  2019-09-06       Impact factor: 2.670

10.  Ionotropic receptors signal host recognition in the salmon louse (Lepeophtheirus salmonis, Copepoda).

Authors:  Anna Z Komisarczuk; Sindre Grotmol; Frank Nilsen
Journal:  PLoS One       Date:  2017-06-05       Impact factor: 3.240

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  3 in total

1.  Proximity ligation strategy for the genomic reconstruction of microbial communities associated with the ectoparasite Caligus rogercresseyi.

Authors:  Diego Valenzuela-Miranda; Ana Teresa Gonçalves; Valentina Valenzuela-Muñoz; Gustavo Nuñez-Acuña; Ivan Liachko; Bradley Nelson; Cristian Gallardo-Escarate
Journal:  Sci Rep       Date:  2022-01-17       Impact factor: 4.996

2.  Exploring Sea Lice Vaccines against Early Stages of Infestation in Atlantic Salmon (Salmo salar).

Authors:  Antonio Casuso; Valentina Valenzuela-Muñoz; Bárbara P Benavente; Diego Valenzuela-Miranda; Cristian Gallardo-Escárate
Journal:  Vaccines (Basel)       Date:  2022-07-01

3.  Trypsin Genes Are Regulated through the miRNA Bantam and Associated with Drug Sensitivity in the Sea Louse Caligus rogercresseyi.

Authors:  Gustavo Núñez-Acuña; Valentina Valenzuela-Muñoz; Crisleri Carrera-Naipil; Constanza Sáez-Vera; Bárbara P Benavente; Diego Valenzuela-Miranda; Cristian Gallardo-Escárate
Journal:  Noncoding RNA       Date:  2021-12-03
  3 in total

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