Mohammad Azam Ansari1, Sarah Mousa Maadi Asiri2, Mohammad A Alzohairy3, Mohammad N Alomary4, Ahmad Almatroudi3, Firdos Alam Khan5. 1. Department of Epidemic Disease Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia. 2. Department of Biophysics, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia. 3. Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Qassim University, Qassim 51431, Saudi Arabia. 4. National Center for Biotechnology, Life Science and Environmental Research Institute, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11451, Saudi Arabia. 5. Department of Stem Cell Biology, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia.
Abstract
The current study demonstrates the synthesis of fatty acids (FAs) capped silver nanoparticles (AgNPs) using aqueous poly-herbal drug Liv52 extract (PLE) as a reducing, dispersing and stabilizing agent. The NPs were characterized by various techniques and used to investigate their potent antibacterial, antibiofilm, antifungal and anticancer activities. GC-MS analysis of PLE shows a total of 37 peaks for a variety of bio-actives compounds. Amongst them, n-hexadecanoic acid (21.95%), linoleic acid (20.45%), oleic acid (18.01%) and stearic acid (13.99%) were found predominately and most likely acted as reducing, stabilizing and encapsulation FAs in LIV-AgNPs formation. FTIR analysis of LIV-AgNPs shows some other functional bio-actives like proteins, sugars and alkenes in the soft PLE corona. The zone of inhibition was 10.0 ± 2.2-18.5 ± 1.0 mm, 10.5 ± 2.5-22.5 ± 1.5 mm and 13.7 ± 1.0-16.5 ± 1.2 against P. aeruginosa, S. aureus and C. albicans, respectively. LIV-AgNPs inhibit biofilm formation in a dose-dependent manner i.e., 54.4% ± 3.1%-10.12% ± 2.3% (S. aureus), 72.7% ± 2.2%-23.3% ± 5.2% (P. aeruginosa) and 85.4% ± 3.3%-25.6% ± 2.2% (C. albicans), and SEM analysis of treated planktonic cells and their biofilm biomass validated the fitness of LIV-AgNPs in future nanoantibiotics. In addition, as prepared FAs rich PLE capped AgNPs have also exhibited significant (p < 0.05 *) antiproliferative activity against cultured HCT-116 cells. Overall, this is a very first demonstration on employment of FAs rich PLE for the synthesis of highly dispersible, stable and uniform sized AgNPs and their antibacterial, antifungal, antibiofilm and anticancer efficacy.
The current study demonstrates the synthesis of pan class="Chemical">fatty acids (FAs) capped silver nanoparticles (AgNPs) using aqueous poly-herbal drug Liv52 extract (PLE) as a reducing, dispersing and stabilizing agent. The NPs were characterized by various techniques and used to investigate their potent antibacterial, antibiofilm, antifungal and anticancer activities. GC-MS analysis of PLE shows a total of 37 peaks for a variety of bio-actives compounds. Amongst them, n-hexadecanoic acid (21.95%), linoleic acid (20.45%), oleic acid (18.01%) and stearic acid (13.99%) were found predominately and most likely acted as reducing, stabilizing and encapsulation FAs in LIV-AgNPs formation. FTIR analysis of LIV-AgNPs shows some other functional bio-actives like proteins, sugars and alkenes in the soft PLE corona. The zone of inhibition was 10.0 ± 2.2-18.5 ± 1.0 mm, 10.5 ± 2.5-22.5 ± 1.5 mm and 13.7 ± 1.0-16.5 ± 1.2 against P. aeruginosa, S. aureus and C. albicans, respectively. LIV-AgNPs inhibit biofilm formation in a dose-dependent manner i.e., 54.4% ± 3.1%-10.12% ± 2.3% (S. aureus), 72.7% ± 2.2%-23.3% ± 5.2% (P. aeruginosa) and 85.4% ± 3.3%-25.6% ± 2.2% (C. albicans), and SEM analysis of treated planktonic cells and their biofilm biomass validated the fitness of LIV-AgNPs in future nanoantibiotics. In addition, as prepared FAs rich PLE capped AgNPs have also exhibited significant (p < 0.05 *) antiproliferative activity against cultured HCT-116 cells. Overall, this is a very first demonstration on employment of FAs rich PLE for the synthesis of highly dispersible, stable and uniform sized AgNPs and their antibacterial, antifungal, antibiofilm and anticancer efficacy.
Entities:
Keywords:
FT-IR; GC-MS; XRD; biofilm; colon cancer; drug resistant; fatty acids; metal nanoparticles; polyherbal drug
Authors: Ahmed I El-Batal; M Abd Elkodous; Gharieb S El-Sayyad; Nawal E Al-Hazmi; Mohamed Gobara; Ahmad Baraka Journal: Int J Biol Macromol Date: 2020-09-25 Impact factor: 6.953
Authors: Kamlesh A Soni; Palmy Jesudhasan; Martha Cepeda; Kenneth Widmer; G K Jayaprakasha; Bhimanagouda S Patil; Michael E Hume; Suresh D Pillai Journal: J Food Prot Date: 2008-01 Impact factor: 2.077
Authors: Syed Ghazanfar Ali; Mohammad Azam Ansari; Mohammad A Alzohairy; Mohammad N Alomary; Sami AlYahya; Mohammad Jalal; Haris M Khan; Sarah Mousa Maadi Asiri; Wasim Ahmad; Abbas Ali Mahdi; Ahmed M El-Sherbeeny; Mohammed A El-Meligy Journal: Antibiotics (Basel) Date: 2020-02-27
Authors: Kollur Shiva Prasad; Shashanka K Prasad; Mohammad Azam Ansari; Mohammad A Alzohairy; Mohammad N Alomary; Sami AlYahya; Chandrashekar Srinivasa; Mahadevamurthy Murali; Veena Malligere Ankegowda; Chandan Shivamallu Journal: Biomolecules Date: 2020-06-30
Authors: Mohammad Azam Ansari; Sultan Akhtar; Mohd Ahmar Rauf; Mohammad N Alomary; Sami AlYahya; Saad Alghamdi; M A Almessiere; Abdulhadi Baykal; Firdos Khan; Syed Farooq Adil; Mujeeb Khan; Mohammad Rafe Hatshan Journal: Int J Nanomedicine Date: 2021-08-18