Literature DB >> 3357116

A new method for cloning Giardia lamblia, with a discussion of the statistical considerations of limiting dilution.

K F Baum1, R L Berens, R H Jones, J J Marr.   

Abstract

This report describes a method of cloning Giardia lamblia by limiting dilution which is simpler than the previously described semisolid agar technique and which may also be applied as an assay of cell viability. A discussion of the basic statistics of limiting dilution, which is applicable to any cell type, and a method of statistically comparing colony-forming efficiencies from different cell populations are included. The colony-forming efficiency (CFE) of this method, when applied to late log-phase cultures, is 72.1 +/- 10.05%. When only cells adherent to the sides of culture vials are cloned, the CFE is 87.1 +/- 9.85%.

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Year:  1988        PMID： 3357116

Source DB:  PubMed          Journal:  J Parasitol        ISSN： 0022-3395            Impact factor:   1.276


  10 in total

1.  Isolation of two Giardia lamblia (WB strain) clones with distinct surface protein and antigenic profiles and differing infectivity and virulence.

Authors:  I A Udezulu; G S Visvesvara; D M Moss; G J Leitch
Journal:  Infect Immun       Date:  1992-06       Impact factor: 3.441

2.  Nuclear inheritance and genetic exchange without meiosis in the binucleate parasite Giardia intestinalis.

Authors:  Meredith L Carpenter; Zoe June Assaf; Stéphane Gourguechon; W Zacheus Cande
Journal:  J Cell Sci       Date:  2012-02-24       Impact factor: 5.285

3.  Frequent rearrangements of rRNA-encoding chromosomes in Giardia lamblia.

Authors:  S M Le Blancq; S H Korman; L H Van der Ploeg
Journal:  Nucleic Acids Res       Date:  1991-08-25       Impact factor: 16.971

4.  Antigenic switching of TSA 417, a trophozoite variable surface protein, following completion of the life cycle of Giardia lamblia.

Authors:  T C Meng; M L Hetsko; F D Gillin
Journal:  Infect Immun       Date:  1993-12       Impact factor: 3.441

5.  Investigation of human giardiasis by karyotype analysis.

Authors:  S H Korman; S M Le Blancq; R J Deckelbaum; L H Van der Ploeg
Journal:  J Clin Invest       Date:  1992-06       Impact factor: 14.808

6.  Genotypic heterogeneity within Giardia lamblia isolates demonstrated by M13 DNA fingerprinting.

Authors:  S Carnaby; P H Katelaris; A Naeem; M J Farthing
Journal:  Infect Immun       Date:  1994-05       Impact factor: 3.441

7.  Spontaneous chromosome rearrangements in the protozoan Giardia lamblia: estimation of mutation rates.

Authors:  S M Le Blancq; S H Korman; L H Van der Ploeg
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

8.  Attachment of Giardia lamblia trophozoites to a cultured human intestinal cell line.

Authors:  P H Katelaris; A Naeem; M J Farthing
Journal:  Gut       Date:  1995-10       Impact factor: 23.059

Review 9.  Uveal melanoma.

Authors:  Martine J Jager; Carol L Shields; Colleen M Cebulla; Mohamed H Abdel-Rahman; Hans E Grossniklaus; Marc-Henri Stern; Richard D Carvajal; Rubens N Belfort; Renbing Jia; Jerry A Shields; Bertil E Damato
Journal:  Nat Rev Dis Primers       Date:  2020-04-09       Impact factor: 52.329

10.  Allelic sequence heterozygosity in single Giardia parasites.

Authors:  Johan Ankarklev; Staffan G Svärd; Marianne Lebbad
Journal:  BMC Microbiol       Date:  2012-05-03       Impact factor: 3.605
  10 in total

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