Nabila Benamrouche1, Badia Guettou2, Fatma Zohra Henniche3, Farida Assaous2, Houcine Laouar4, Hanifa Ziane5, Fazia Djennane5, Djamal Tiouit3, Chafia Bentchouala4, Ferroudja Yamouni6, Kheira Rahal2, Hassiba Tali Maamar2. 1. Medical Bacteriology Laboratory, Institut Pasteur, Algiers, Algeria. n_benamrouche@yahoo.fr. 2. Medical Bacteriology Laboratory, Institut Pasteur, Algiers, Algeria. 3. Microbiology Laboratory, Mohamed Seghir Nekkache Central Hospital of Army, Algiers, Algeria. 4. Department of Microbiology, University Hospital of Constantine, Constantine, Algeria. 5. Department of Microbiology, Mustapha University Hospital, Algiers, Algeria. 6. Central Laboratory, University Regional Military Hospital of Oran, Oran, Algeria.
Abstract
INTRODUCTION: vancomycin-resistant Enterococcus faecium (VREfm) is a major public health problem worldwide. The aim of our study was to determine the microbiological, epidemiological and molecular characteristics of VREfm isolated in north-central, eastern and western Algeria. METHODOLOGY: a collection of 48 VREfm isolated from September 2010 to April 2017 in several Algerian hospitals were studied. Minimum inhibitory concentrations (MICs) were determined by E-test method according to CLSI guidelines. the detection of van genotype of all strains was performed by PCR. Clonal relationship of five VREfm targeted by region were characterized using multilocus sequence typing (MLST). RESULTS: All isolates have multidrug-resistance (MDR) and were resistant to at least five classes of antibiotics; however, all were susceptible to tigecycline and daptomycin with MIC50 at 0.094 µg/mL and 2 µg/mL respectively. All strains belonged to vanA genotype and have high level of resistance to vancomycin and teicoplanin. MLST revealed two sequence types (STs): ST80 (from the four regions of Algeria) and ST789, both belonging to the former hospital-adapted clonal complex CC17. CONCLUSIONS: the alarming dissemination of MDR E. faecium vanA and the ST80 in several regions of Algeria suggest a clonal spread of VREfm strains, which urgently require implementation of adequate infection control measures. Copyright (c) 2021 Nabila Benamrouche, Badia Guettou, Fatma Zohra Henniche, Farida Assaous, Houcine Laouar, Hanifa Ziane, Fazia Djennane, Djamal Tiouit, Chafia Bentchouala, Ferroudja Yamouni, Kheira Rahal, Hassiba Tali Maamar.
INTRODUCTION:vancomycin-resistant Enterococcus faecium (VREfm) is a major public health problem worldwide. The aim of our study was to determine the microbiological, epidemiological and molecular characteristics of VREfm isolated in north-central, eastern and western Algeria. METHODOLOGY: a collection of 48 VREfm isolated from September 2010 to April 2017 in several Algerian hospitals were studied. Minimum inhibitory concentrations (MICs) were determined by E-test method according to CLSI guidelines. the detection of van genotype of all strains was performed by PCR. Clonal relationship of five VREfm targeted by region were characterized using multilocus sequence typing (MLST). RESULTS: All isolates have multidrug-resistance (MDR) and were resistant to at least five classes of antibiotics; however, all were susceptible to tigecycline and daptomycin with MIC50 at 0.094 µg/mL and 2 µg/mL respectively. All strains belonged to vanA genotype and have high level of resistance to vancomycin and teicoplanin. MLST revealed two sequence types (STs): ST80 (from the four regions of Algeria) and ST789, both belonging to the former hospital-adapted clonal complex CC17. CONCLUSIONS: the alarming dissemination of MDR E. faecium vanA and the ST80 in several regions of Algeria suggest a clonal spread of VREfm strains, which urgently require implementation of adequate infection control measures. Copyright (c) 2021 Nabila Benamrouche, Badia Guettou, Fatma Zohra Henniche, Farida Assaous, Houcine Laouar, Hanifa Ziane, Fazia Djennane, Djamal Tiouit, Chafia Bentchouala, Ferroudja Yamouni, Kheira Rahal, Hassiba Tali Maamar.