Won-Hyuk Choi1,2, Yong-Deok Kim1,3, Jae-Min Song4,5,6, Sang-Hun Shin7,8. 1. Department of Oral and maxillofacial surgery, School of dentistry, Pusan National University, Yangsan, 50612, Republic of Korea. 2. Department of Oral and maxillofacial surgery, Pusan National University Hospital, Pusan, 49241, Republic of Korea. 3. Dental and Life Science Institute & Dental Research Institute, Pusan National University Dental Hospital, Yangsan, 50612, Republic of Korea. 4. Department of Oral and maxillofacial surgery, School of dentistry, Pusan National University, Yangsan, 50612, Republic of Korea. songjm@pusan.ac.kr. 5. Department of Oral and maxillofacial surgery, Pusan National University Hospital, Pusan, 49241, Republic of Korea. songjm@pusan.ac.kr. 6. Biomedical Research Institute, Pusan National University Hospital, Pusan, 49241, Republic of Korea. songjm@pusan.ac.kr. 7. Department of Oral and maxillofacial surgery, School of dentistry, Pusan National University, Yangsan, 50612, Republic of Korea. ssh8080@pusan.ac.kr. 8. Dental and Life Science Institute & Dental Research Institute, Pusan National University Dental Hospital, Yangsan, 50612, Republic of Korea. ssh8080@pusan.ac.kr.
Abstract
BACKGROUND: Stability of the grafted bone volume is one of the important factors to the success of alveolar bone grafts. For this, platelet-rich plasma (PRP) or fibrin sealant is mixed with the bone graft material. Bio-Oss® is a protein-free bovine mineral commonly used in bone graft procedures. The grafting particles are commonly combined with a standard fibrin sealant (Tisseel®) to fabricate a plastic implantable product. The purpose of this experiment was to evaluate the efficacy of fibrin sealant (Tisseel®) in bone regeneration performance in a rabbit maxillary sinus model. METHODS: A total of five 3.5 kg weight New Zealand white rabbits were used for the study. After elevating the sinus membrane in both maxillary sinus cavities, Bio-Oss® mixed with normal saline (group 1) was filled into the right side, and Tisseel® mixed Bio-Oss® (group 2) was inserted into the other side. The bone mineral density and bone volume were analyzed with microscopic computed tomography (micro-CT) and histomorphometric 12 weeks after application. RESULTS: Histologically, new bone formation rate was 14.8%, and grafted bone rate was 70.5% in group 1. In group 2, they were 18.5% and 60.4%, respectively. According to micro-CT analysis, bone mineral density (mg/cm3, BMD) was 2.5% larger in group 1. CONCLUSIONS: The findings from this study suggest that, although the difference in the bone formation between group 1 and group 2 appears to be insignificant, group 2 had an advantage in using smaller amount of bone substances to achieve the reliable bone formation.
BACKGROUND: Stability of the grafted bone volume is one of the important factors to the success of alveolar bone grafts. For this, platelet-rich plasma (PRP) or fibrin sealant is mixed with the bone graft material. Bio-Oss® is a protein-free bovine mineral commonly used in bone graft procedures. The grafting particles are commonly combined with a standard fibrin sealant (Tisseel®) to fabricate a plastic implantable product. The purpose of this experiment was to evaluate the efficacy of fibrin sealant (Tisseel®) in bone regeneration performance in a rabbit maxillary sinus model. METHODS: A total of five 3.5 kg weight New Zealand white rabbits were used for the study. After elevating the sinus membrane in both maxillary sinus cavities, Bio-Oss® mixed with normal saline (group 1) was filled into the right side, and Tisseel® mixed Bio-Oss® (group 2) was inserted into the other side. The bone mineral density and bone volume were analyzed with microscopic computed tomography (micro-CT) and histomorphometric 12 weeks after application. RESULTS: Histologically, new bone formation rate was 14.8%, and grafted bone rate was 70.5% in group 1. In group 2, they were 18.5% and 60.4%, respectively. According to micro-CT analysis, bone mineral density (mg/cm3, BMD) was 2.5% larger in group 1. CONCLUSIONS: The findings from this study suggest that, although the difference in the bone formation between group 1 and group 2 appears to be insignificant, group 2 had an advantage in using smaller amount of bone substances to achieve the reliable bone formation.
Entities:
Keywords:
Animal models; Bone graft; Fibrin sealant; Maxillary sinus; Xenograft