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Literature DB >> 3356459

Identification of the major antigens of Treponema hyodysenteriae and comparison with those of Treponema innocens.

S N Chatfield¹, D S Fernie, C Penn, G Dougan.

Abstract

Eleven strains of Treponema hyodysenteriae isolated from pigs with swine dysentery were examined by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. T. hyodysenteriae strains formed a homogeneous group with respect to sodium dodecyl sulfate-soluble proteins. However, immunoblotting with antiserum from rabbits immunized with T. hyodysenteriae CN8368 revealed heterogeneity among the lipopolysaccharide complexes of different strains. Polypeptides of molecular weights between 30,000 and 36,000 were the predominant T. hyodysenteriae polypeptides detected by porcine immune serum. In contrast, Treponema innocens did not form a homogeneous group with respect to sodium dodecyl sulfate-soluble proteins. Adsorption studies and immunoblotting identified polypeptide antigens present on cells of T. hyodysenteriae which were not detected on cells of T. innocens. These unique antigens may play a role in the virulence of T. hyodysenteriae.

Entities: Chemical Species

Mesh：

Substances：
Antigens, Bacterial

Year: 1988 PMID： 3356459 PMCID： PMC259764 DOI： 10.1128/iai.56.5.1070-1075.1988

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

22 in total

1. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

2. Enzyme-linked immunosorbent assay for detection of antibody to Treponema hyodysenteriae antigens.

Authors: L A Joens; N A Nord; J M Kinyon; I T Egan
Journal: J Clin Microbiol Date: 1982-02 Impact factor: 5.948

3. Serotype-specific protection against Treponema hyodysenteriae infection in ligated colonic loops of pigs recovered from swine dysentery.

Authors: L A Joens; S C Whipp; R D Glock; M E Neussen
Journal: Infect Immun Date: 1983-01 Impact factor: 3.441

4. Swine dysentery: protection against experimental challenge following single dose parenteral immunisation with inactivated Treponema hyodysenteriae.

Authors: D S Fernie; P H Ripley; P D Walker
Journal: Res Vet Sci Date: 1983-09 Impact factor: 2.534

5. A study of swine dysentery by immunofluorescence and histology.

Authors: R Hughes; H J Olander; D L Kanitz; S Qureshi
Journal: Vet Pathol Date: 1977-09 Impact factor: 2.221

6. Biological activity of a lipopolysaccharide extracted from Treponema hyodysenteriae.

Authors: M E Nuessen; J R Birmingham; L A Joens
Journal: Infect Immun Date: 1982-07 Impact factor: 3.441

7. Involvement of lipopolysaccharide in the pathogenicity of Treponema hyodysenteriae.

Authors: M E Nuessen; L A Joens; R D Glock
Journal: J Immunol Date: 1983-08 Impact factor: 5.422

8. Immunity to Swine dysentery in recovered pigs.

Authors: L A Joens; D L Harris; D H Baum
Journal: Am J Vet Res Date: 1979-10 Impact factor: 1.156

9. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels.

Authors: P J Hitchcock; T M Brown
Journal: J Bacteriol Date: 1983-04 Impact factor: 3.490

10. Enteropathogenicity of various isolates of Treponema hyodysenteriae.

Authors: J M Kinyon; D L Harris; R D Glock
Journal: Infect Immun Date: 1977-02 Impact factor: 3.441

11 in total

1. Characterization of the major outer membrane antigens of Treponema hyodysenteriae.

Authors: M J Wannemuehler; R D Hubbard; J M Greer
Journal: Infect Immun Date: 1988-12 Impact factor: 3.441

2. Analysis of lipopolysaccharide antigens of Treponema hyodysenteriae.

Authors: D J Hampson; J R Mhoma; B Combs
Journal: Epidemiol Infect Date: 1989-10 Impact factor: 2.451

3. Cloning and DNA sequence analysis of a Serpulina (Treponema) hyodysenteriae gene encoding a periplasmic flagellar sheath protein.

Authors: M B Koopman; O S de Leeuw; B M van der Zeijst; J G Kusters
Journal: Infect Immun Date: 1992-07 Impact factor: 3.441

4. Purified outer membranes of Serpulina hyodysenteriae contain cholesterol.

Authors: H Plaza; T R Whelchel; S F Garczynski; E W Howerth; F C Gherardini
Journal: J Bacteriol Date: 1997-09 Impact factor: 3.490

5. Development of an experimental model allowing discrimination between virulent and avirulent isolates of Serpulina (Treponema) hyodysenteriae.

Authors: M Achacha; S Messier; K R Mittal
Journal: Can J Vet Res Date: 1996-01 Impact factor: 1.310

6. Application and evaluation of enzyme-linked immunosorbent assay and immunoblotting for detection of antibodies to Treponema hyodysenteriae in swine.

Authors: S C Smith; L M Barrett; T Muir; W L Christopher; P J Coloe
Journal: Epidemiol Infect Date: 1991-10 Impact factor: 2.451