E Bandariyan1, A Mogheiseh2, A Ahmadi3. 1. Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, P.O. Box. 7144169155, Shiraz, Fars, Iran. 2. Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, P.O. Box. 7144169155, Shiraz, Fars, Iran. mogheiseh@yahoo.com. 3. Department of Basic Sciences, School of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran.
Abstract
BACKGROUND: Polycystic ovary syndrome (PCOS) is one of the most prevalent endocrinopathies in women during the reproductive age. Herbal medicines are used increasingly alone or in supplement with chemical medicines for the treatment of different diseases and dysfunctions. This study was aimed to evaluate the effects of lutein and nettle (Urtica dioica) extract on the biochemical parameters and the reproductive function in the PCOS model of mice. METHODS: Following the induction of PCOS by dehydroepiandrosterone (DHEA), the mice (n = 98) were randomly assigned into seven groups, each consisting of fourteen mice; the groups were included control group (received solvent), PCOS group (received 6 mg/100 g B.W/day IP, DHEA for 21 days), PCOS+ Nettle extract (200 and 400 mg/kg), PCOS+ Lutein (125 and 250 mg/kg), and PCOS+ NL (200 mg/kg nettle extract and 125 mg/kg lutein). The nettle extract and lutein were administrated using gavage for 30 consecutive days after PCOS induction. Malondialdehyde (MDA), total antioxidant capacity (TAC), and estrogen were measured in serum, ovary, and uterus samples by the ELISA method. The total number of oocytes, oocyte quality, fertilization rate, 2-cell blastocyst, and arrested embryos (type I, type II, and type III) were also investigated. RESULTS: A combination treatment of the nettle and lutein produced the lowest concentration of MDA in comparison to other groups which affected by the PCOS. The lowest level of TAC was observed in the PCOS group without treatment. The number of oocytes, oocyte quality, fertilization rate, and 2-cell blastocyst were significantly higher in the control group, but the lowest values were observed in the PCOS group without any treatment. CONCLUSIONS: The most favorable findings include improving antioxidant capacity, oocyte and embryo quality were observed in the PCOS+ 125 L group.
BACKGROUND:Polycystic ovary syndrome (PCOS) is one of the most prevalent endocrinopathies in women during the reproductive age. Herbal medicines are used increasingly alone or in supplement with chemical medicines for the treatment of different diseases and dysfunctions. This study was aimed to evaluate the effects of lutein and nettle (Urtica dioica) extract on the biochemical parameters and the reproductive function in the PCOS model of mice. METHODS: Following the induction of PCOS by dehydroepiandrosterone (DHEA), the mice (n = 98) were randomly assigned into seven groups, each consisting of fourteen mice; the groups were included control group (received solvent), PCOS group (received 6 mg/100 g B.W/day IP, DHEA for 21 days), PCOS+ Nettle extract (200 and 400 mg/kg), PCOS+ Lutein (125 and 250 mg/kg), and PCOS+ NL (200 mg/kg nettle extract and 125 mg/kg lutein). The nettle extract and lutein were administrated using gavage for 30 consecutive days after PCOS induction. Malondialdehyde (MDA), total antioxidant capacity (TAC), and estrogen were measured in serum, ovary, and uterus samples by the ELISA method. The total number of oocytes, oocyte quality, fertilization rate, 2-cell blastocyst, and arrested embryos (type I, type II, and type III) were also investigated. RESULTS: A combination treatment of the nettle and lutein produced the lowest concentration of MDA in comparison to other groups which affected by the PCOS. The lowest level of TAC was observed in the PCOS group without treatment. The number of oocytes, oocyte quality, fertilization rate, and 2-cell blastocyst were significantly higher in the control group, but the lowest values were observed in the PCOS group without any treatment. CONCLUSIONS: The most favorable findings include improving antioxidant capacity, oocyte and embryo quality were observed in the PCOS+ 125 L group.
Authors: F Gaytán; M Gaytán; J M Castellano; M Romero; J Roa; B Aparicio; N Garrido; J E Sánchez-Criado; R P Millar; A Pellicer; H M Fraser; M Tena-Sempere Journal: Am J Physiol Endocrinol Metab Date: 2009-01-13 Impact factor: 4.310
Authors: Helena J Teede; Marie L Misso; Jacqueline A Boyle; Rhonda M Garad; Veryan McAllister; Linda Downes; Melanie Gibson; Roger J Hart; Luk Rombauts; Lisa Moran; Anuja Dokras; Joop Laven; Terhi Piltonen; Raymond J Rodgers; Mala Thondan; Michael F Costello; Robert J Norman Journal: Med J Aust Date: 2018-10-01 Impact factor: 7.738
Authors: Olga Krystofova; Vojtech Adam; Petr Babula; Josef Zehnalek; Miroslava Beklova; Ladislav Havel; Rene Kizek Journal: Int J Environ Res Public Health Date: 2010-10-22 Impact factor: 3.390
Authors: Richard S Legro; Silva A Arslanian; David A Ehrmann; Kathleen M Hoeger; M Hassan Murad; Renato Pasquali; Corrine K Welt Journal: J Clin Endocrinol Metab Date: 2013-10-22 Impact factor: 5.958
Authors: V De Leo; M C Musacchio; V Cappelli; M G Massaro; G Morgante; F Petraglia Journal: Reprod Biol Endocrinol Date: 2016-07-16 Impact factor: 5.211