Fangfang Qiao1, Xiwen Yang1, Fengdan Xu1, Yuan Huang1, Jiemei Zhang1, Miao Song1, Sumei Zhou1, Meng Zhang2, Dexian He3. 1. College of Agronomy, Henan Agricultural University/ National Engineering Research Center for Wheat/ Co-construction State Key Laboratory of Wheat and Maize Crop Science/ Collaborative Innovation Center of Henan Grain Crops, 15 Longzihu College District, Zhengzhou, 450046, China. 2. College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, Henan, China. zm2006@126.com. 3. College of Agronomy, Henan Agricultural University/ National Engineering Research Center for Wheat/ Co-construction State Key Laboratory of Wheat and Maize Crop Science/ Collaborative Innovation Center of Henan Grain Crops, 15 Longzihu College District, Zhengzhou, 450046, China. hed@henau.edu.cn.
Abstract
BACKGROUND: Fusarium crown rot is major disease in wheat. However, the wheat defense mechanisms against this disease remain poorly understood. RESULTS: Using tandem mass tag (TMT) quantitative proteomics, we evaluated a disease-susceptible (UC1110) and a disease-tolerant (PI610750) wheat cultivar inoculated with Fusarium pseudograminearum WZ-8A. The morphological and physiological results showed that the average root diameter and malondialdehyde content in the roots of PI610750 decreased 3 days post-inoculation (dpi), while the average number of root tips increased. Root vigor was significantly increased in both cultivars, indicating that the morphological, physiological, and biochemical responses of the roots to disease differed between the two cultivars. TMT analysis showed that 366 differentially expressed proteins (DEPs) were identified by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment in the two comparison groups, UC1110_3dpi/UC1110_0dpi (163) and PI610750_3dpi/PI610750_0dpi (203). It may be concluded that phenylpropanoid biosynthesis (8), secondary metabolite biosynthesis (12), linolenic acid metabolites (5), glutathione metabolism (8), plant hormone signal transduction (3), MAPK signaling pathway-plant (4), and photosynthesis (12) contributed to the defense mechanisms in wheat. Protein-protein interaction network analysis showed that the DEPs interacted in both sugar metabolism and photosynthesis pathways. Sixteen genes were validated by real-time quantitative polymerase chain reaction and were found to be consistent with the proteomics data. CONCLUSION: The results provided insight into the molecular mechanisms of the interaction between wheat and F. pseudograminearum.
BACKGROUND:Fusarium crown rot is major disease in wheat. However, the wheat defense mechanisms against this disease remain poorly understood. RESULTS: Using tandem mass tag (TMT) quantitative proteomics, we evaluated a disease-susceptible (UC1110) and a disease-tolerant (PI610750) wheat cultivar inoculated with Fusarium pseudograminearumWZ-8A. The morphological and physiological results showed that the average root diameter and malondialdehyde content in the roots of PI610750 decreased 3 days post-inoculation (dpi), while the average number of root tips increased. Root vigor was significantly increased in both cultivars, indicating that the morphological, physiological, and biochemical responses of the roots to disease differed between the two cultivars. TMT analysis showed that 366 differentially expressed proteins (DEPs) were identified by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment in the two comparison groups, UC1110_3dpi/UC1110_0dpi (163) and PI610750_3dpi/PI610750_0dpi (203). It may be concluded that phenylpropanoid biosynthesis (8), secondary metabolite biosynthesis (12), linolenic acid metabolites (5), glutathione metabolism (8), plant hormone signal transduction (3), MAPK signaling pathway-plant (4), and photosynthesis (12) contributed to the defense mechanisms in wheat. Protein-protein interaction network analysis showed that the DEPs interacted in both sugar metabolism and photosynthesis pathways. Sixteen genes were validated by real-time quantitative polymerase chain reaction and were found to be consistent with the proteomics data. CONCLUSION: The results provided insight into the molecular mechanisms of the interaction between wheat and F. pseudograminearum.
Authors: Jens Laurids Sørensen; Aurelie H Benfield; Rasmus Dam Wollenberg; Klaus Westphal; Reinhard Wimmer; Mikkel Rank Nielsen; Kristian Fog Nielsen; Jason Carere; Lorenzo Covarelli; Giovanni Beccari; Jonathan Powell; Takafumi Yamashino; Herbert Kogler; Teis Esben Sondergaard; Donald Max Gardiner Journal: Mol Plant Pathol Date: 2017-10-24 Impact factor: 5.663
Authors: S Restrepo; K L Myers; O del Pozo; G B Martin; A L Hart; C R Buell; W E Fry; C D Smart Journal: Mol Plant Microbe Interact Date: 2005-09 Impact factor: 4.171
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