Karina M Tonon1, Tania B Morais2, Carla R Taddei3,4, Humberto B Araújo-Filho5, Ana Cristina F V Abrão6, Antonio Miranda7, Mauro B de Morais5. 1. Nutrition Postgraduate Program, Universidade Federal de São Paulo, São Paulo, Brazil. 2. Food Quality Control Laboratory, Universidade Federal de São Paulo, São Paulo, Brazil. 3. Department of Clinical and Toxicological Analysis, Universidade de São Paulo, São Paulo, Brazil. 4. School of Arts, Sciences and Humanities, Universidade de São Paulo, São Paulo, Brazil. 5. Division of Pediatric Gastroenterology, Universidade Federal de São Paulo, São Paulo, Brazil. 6. Breastfeeding Incentive and Support Center, Universidade Federal de São Paulo, São Paulo, Brazil. 7. Department of Biophysics, Universidade Federal de São Paulo, São Paulo, Brazil.
Abstract
BACKGROUND: Exclusive breastfeeding promotes beneficial modifications on the microbiota of cesarean born infants, but little is known about the role of specific breast milk components in this modulation. Women with an active FUT2 gene (called secretors) secrete α1-2 fucosylated human milk oligosaccharides (HMOs), which promote Bifidobacterium in the infant's gut and may modulate the microbiota of cesarean born infants. OBJECTIVE: To compare the microbiota composition of cesarean and vaginally born infants breastfed by secretor mothers. METHODS: Maternal secretor status was determined by the occurrence of 4 different α1-2 fucosylated HMOs in breast milk by LC-MS. The fecal microbiota composition from cesarean and vaginally born infants was analyzed by 16S rRNA gene sequencing and qPCR, stratified by the maternal secretor status, and compared. RESULTS: Alpha and beta diversity were not significantly different in cesarean born, secretor-fed infants (CSe+) compared to vaginally born, secretor-fed infants (VSe+). There were no significant differences in the fecal relative abundance of Bifidobacterium between CSe+ and VSe+ infants, but the prevalence of the species B. longum was lower in CSe+. The fecal relative abundance of Bacteroides was also lower, while Akkermansia and Kluyvera were higher in CSe+ infants. CONCLUSION: Cesarean and vaginally born infants fed with breast milk containing the α1-2 fucosylated HMOs fraction present similar amounts of Bifidobacterium in the feces, but differences are observed in other members of the microbiota.
BACKGROUND: Exclusive breastfeeding promotes beneficial modifications on the microbiota of cesarean born infants, but little is known about the role of specific breast milk components in this modulation. Women with an active FUT2 gene (called secretors) secrete α1-2 fucosylated human milk oligosaccharides (HMOs), which promote Bifidobacterium in the infant's gut and may modulate the microbiota of cesarean born infants. OBJECTIVE: To compare the microbiota composition of cesarean and vaginally born infants breastfed by secretor mothers. METHODS: Maternal secretor status was determined by the occurrence of 4 different α1-2 fucosylated HMOs in breast milk by LC-MS. The fecal microbiota composition from cesarean and vaginally born infants was analyzed by 16S rRNA gene sequencing and qPCR, stratified by the maternal secretor status, and compared. RESULTS: Alpha and beta diversity were not significantly different in cesarean born, secretor-fed infants (CSe+) compared to vaginally born, secretor-fed infants (VSe+). There were no significant differences in the fecal relative abundance of Bifidobacterium between CSe+ and VSe+ infants, but the prevalence of the species B. longum was lower in CSe+. The fecal relative abundance of Bacteroides was also lower, while Akkermansia and Kluyvera were higher in CSe+ infants. CONCLUSION: Cesarean and vaginally born infants fed with breast milk containing the α1-2 fucosylated HMOs fraction present similar amounts of Bifidobacterium in the feces, but differences are observed in other members of the microbiota.
Authors: James J Kozich; Sarah L Westcott; Nielson T Baxter; Sarah K Highlander; Patrick D Schloss Journal: Appl Environ Microbiol Date: 2013-06-21 Impact factor: 4.792
Authors: Hein M Tun; Sarah L Bridgman; Radha Chari; Catherine J Field; David S Guttman; Allan B Becker; Piush J Mandhane; Stuart E Turvey; Padmaja Subbarao; Malcolm R Sears; James A Scott; Anita L Kozyrskyj Journal: JAMA Pediatr Date: 2018-04-01 Impact factor: 16.193
Authors: Karina M Tonon; Antonio Miranda; Ana Cristina F V Abrão; Mauro B de Morais; Tania B Morais Journal: Food Chem Date: 2018-09-05 Impact factor: 7.514
Authors: Zachery T Lewis; Sarah M Totten; Jennifer T Smilowitz; Mina Popovic; Evan Parker; Danielle G Lemay; Maxwell L Van Tassell; Michael J Miller; Yong-Su Jin; J Bruce German; Carlito B Lebrilla; David A Mills Journal: Microbiome Date: 2015-04-10 Impact factor: 14.650
Authors: Philip Strandwitz; Ki Hyun Kim; Darya Terekhova; Joanne K Liu; Anukriti Sharma; Jennifer Levering; Daniel McDonald; David Dietrich; Timothy R Ramadhar; Asama Lekbua; Nader Mroue; Conor Liston; Eric J Stewart; Marc J Dubin; Karsten Zengler; Rob Knight; Jack A Gilbert; Jon Clardy; Kim Lewis Journal: Nat Microbiol Date: 2018-12-10 Impact factor: 17.745
Authors: Yan Shao; Samuel C Forster; Evdokia Tsaliki; Kevin Vervier; Angela Strang; Nandi Simpson; Nitin Kumar; Mark D Stares; Alison Rodger; Peter Brocklehurst; Nigel Field; Trevor D Lawley Journal: Nature Date: 2019-09-18 Impact factor: 49.962