Iman Fathy Abou-El-Naga1, Nermine Mogahed Fawzy Hussein Mogahed2. 1. Professor of Parasitology, Faculty of Medicine, Alexandria University, Alexandria, Egypt. 2. Lecturer of Parasitology, Faculty of Medicine, Alexandria University, Alexandria, Egypt. nermine_mogahed@yahoo.com.
Abstract
PURPOSES: Evaluate the effect of auranofin on the early and late stages of chronic infection with Toxoplasma gondii avirulent ME49 strain. METHODS: Swiss albino mice were orally inoculated with 10 cysts of Toxoplasma gondii, and orally treated with auranofin or septazole in daily doses of 20 mg/kg or 100 mg /kg, respectively, for 30 days. Treatment began either on the same day of infection and mice were sacrificed at the 60th day postinfection or the treatment started after 60 days of infection and mice were sacrificed at the 90th day postinfection. RESULTS: Auranofin significantly reduced the brain cyst burden and inflammatory reaction at both stages of infection compared to the infected non-treated control. More remarkably, auranofin significant reduced the brain cyst burden in the late stage, while septazole failed. Hydrogen peroxide level was significantly increased in the brain homogenate of mice treated with auranofin only at the early stage of infection. Ultrastructral studies revealed that the anti-Toxoplasma effect of auranofin is achieved by changing the membrane permeability and inducing apoptosis. CONCLUSIONS: Thus, auranofin could be an alternative for the standard treatment regimen of toxoplasmosis and these results are considered another achievement for the drug against parasitic infection. Being a FDA-approved drug, it can be rapidly evaluated in clinical trials.
PURPOSES: Evaluate the effect of auranofin on the early and late stages of chronic infection with Toxoplasma gondii avirulent ME49 strain. METHODS: Swiss albino mice were orally inoculated with 10 cysts of Toxoplasma gondii, and orally treated with auranofin or septazole in daily doses of 20 mg/kg or 100 mg /kg, respectively, for 30 days. Treatment began either on the same day of infection and mice were sacrificed at the 60th day postinfection or the treatment started after 60 days of infection and mice were sacrificed at the 90th day postinfection. RESULTS:Auranofin significantly reduced the brain cyst burden and inflammatory reaction at both stages of infection compared to the infected non-treated control. More remarkably, auranofin significant reduced the brain cyst burden in the late stage, while septazole failed. Hydrogen peroxide level was significantly increased in the brain homogenate of mice treated with auranofin only at the early stage of infection. Ultrastructral studies revealed that the anti-Toxoplasma effect of auranofin is achieved by changing the membrane permeability and inducing apoptosis. CONCLUSIONS: Thus, auranofin could be an alternative for the standard treatment regimen of toxoplasmosis and these results are considered another achievement for the drug against parasitic infection. Being a FDA-approved drug, it can be rapidly evaluated in clinical trials.
Authors: W A Katz; S Alexander; J H Bland; W Blechman; G B Bluhm; R A Bonebrake; A Falbo; R A Greenwald; S Hartman; T Hobbs; S Indenbaum; J E Lergier; B G Lanier; R W Lightfoot; P Phelps; R P Sheon; D Torretti; M E Wenger; K Wilske Journal: J Rheumatol Suppl Date: 1982 Jul-Aug
Authors: Iman Fathy Abou-El-Naga; Eman Dorry El Kerdany; Rasha Fadly Mady; Thanaa Ibrahim Shalaby; Enas Mohammed Zaytoun Journal: Parasitol Int Date: 2017-08-21 Impact factor: 2.230
Authors: Noa Tejman-Yarden; Yukiko Miyamoto; David Leitsch; Jennifer Santini; Anjan Debnath; Jiri Gut; James H McKerrow; Sharon L Reed; Lars Eckmann Journal: Antimicrob Agents Chemother Date: 2013-02-12 Impact factor: 5.191