Literature DB >> 33554979

cDNA TRAP display for rapid and stable in vitro selection of antibody-like proteins.

Taishi Kondo1, Minori Eguchi, Seita Kito, Tomoshige Fujino, Gosuke Hayashi, Hiroshi Murakami.   

Abstract

We developed a cDNA TRAP display for the rapid selection of antibody-like proteins in various conditions. By modifying the original puromycin linker in the TRAP display, a monobody was covalently attached to the cDNA. As a proof-of-concept, we demonstrated a rapid model selection of an anti-EGFR1 monobody in a solution containing ribonuclease.

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Year:  2021        PMID： 33554979     DOI： 10.1039/d0cc07541h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  3 in total

1.  Interleukin-17A Peptide Aptamers with an Unexpected Binding Moiety Selected by cDNA Display under Heterogenous Conditions.

Authors:  Hiroki Anzai; Takuya Terai; Kanako Wakabayashi-Nakao; Taro Noguchi; Shigefumi Kumachi; Masayuki Tsuchiya; Naoto Nemoto
Journal:  ACS Med Chem Lett       Date:  2021-08-27       Impact factor: 4.632

2.  Monobodies with potent neutralizing activity against SARS-CoV-2 Delta and other variants of concern.

Authors:  Taishi Kondo; Kazuhiro Matsuoka; Shun Umemoto; Tomoshige Fujino; Gosuke Hayashi; Yasumasa Iwatani; Hiroshi Murakami
Journal:  Life Sci Alliance       Date:  2022-03-07

Review 3.  Opportunities for Expanding Encoded Chemical Diversification and Improving Hit Enrichment in mRNA-Displayed Peptide Libraries.

Authors:  Paddy R A Melsen; Ryoji Yoshisada; Seino A K Jongkees
Journal:  Chembiochem       Date:  2022-02-18       Impact factor: 3.461
  3 in total

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