Reza Najafipour1, Abdolmabood Momeni2, Farideh Yousefipour3, Shaghayegh Mousavi4, Sahar Moghbelinejad1. 1. Research Institute for Prevention of Non-Communicable Diseases, Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran. 2. Biology Department, School of Basic Science, Arak University, Arak, Iran. 3. National Institute of Engineering and Biotechnology, Tehran, Iran. 4. Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
Abstract
BACKGROUND: Post-transcriptional microRNAs (miRNAs) have a impotrant pattern in the spermatogenesis process. OBJECTIVE: Study of the expression and methylation of hsa-miR-449 family in sperm samples of infertile men. MATERIALS AND METHODS: In this case-control study, we recruited 74 infertile men (with asthenozoospermia, teratozoospermia, asthenoteratozoospermia, and oligoasthenoteratozoospermia) and 30 control samles. Methylation-specific PCR (MSP) method was used for methylation evaluation of hsa-miR-449 a, b, c promoter. By Real time PCR (qRT-PCR) method,we showed downregulation of hsa-miR-449 a, b, c in the sperm samples of infertile men and compared it to their fertile counterparts. RESULTS: There was significant underexperssion, in hsa-miR-449-b in oligoasthenoteratospermic samples (p = 0.0001, F = 2.9). About the methylation pattern, infertile men showed high frequency of methylation in the promoter of hsa-miR-449 a, b, c in comparison to controls (60.8% vs 23.3%), the highest amount of methylation was observed in oligoasthenoteratospermia samples (81.2%). CONCLUSION: In this study, low expression and high methylation of hsa-miR-449-b were observed in infertile men in compared to control samples, which can be one of the causes of defective spermatogenesis.
BACKGROUND: Post-transcriptional microRNAs (miRNAs) have a impotrant pattern in the spermatogenesis process. OBJECTIVE: Study of the expression and methylation of hsa-miR-449 family in sperm samples of infertile men. MATERIALS AND METHODS: In this case-control study, we recruited 74 infertile men (with asthenozoospermia, teratozoospermia, asthenoteratozoospermia, and oligoasthenoteratozoospermia) and 30 control samles. Methylation-specific PCR (MSP) method was used for methylation evaluation of hsa-miR-449 a, b, c promoter. By Real time PCR (qRT-PCR) method,we showed downregulation of hsa-miR-449 a, b, c in the sperm samples of infertile men and compared it to their fertile counterparts. RESULTS: There was significant underexperssion, in hsa-miR-449-b in oligoasthenoteratospermic samples (p = 0.0001, F = 2.9). About the methylation pattern, infertile men showed high frequency of methylation in the promoter of hsa-miR-449 a, b, c in comparison to controls (60.8% vs 23.3%), the highest amount of methylation was observed in oligoasthenoteratospermia samples (81.2%). CONCLUSION: In this study, low expression and high methylation of hsa-miR-449-b were observed in infertile men in compared to control samples, which can be one of the causes of defective spermatogenesis.
Authors: Qiuxia Wu; Rui Song; Nicole Ortogero; Huili Zheng; Ryan Evanoff; Chris L Small; Michael D Griswold; Satoshi H Namekawa; Helene Royo; James M Turner; Wei Yan Journal: J Biol Chem Date: 2012-06-04 Impact factor: 5.157
Authors: David A Dickson; Jessica K Paulus; Virginia Mensah; Janis Lem; Lorena Saavedra-Rodriguez; Adrienne Gentry; Kelly Pagidas; Larry A Feig Journal: Transl Psychiatry Date: 2018-05-23 Impact factor: 6.222