Chang Liu1, Ruihua Li2, Yang Liu1, Zhenguo Li2, Yujiao Sun1, Peiyuan Yin1,3, Rihong Huang1. 1. First Affiliated Hospital of Dalian Medical University, Dalian, China. 2. Medical Laboratory Science, Second Affiliated Hospital of Dalian Medical University, Dalian, China. 3. College of Integrative Medicine, Dalian Medical University, Dalian, China.
Abstract
Purpose: Metabolic impairment is one key contributor to heart failure (HF) pathogenesis and progression. The major causes of HF, coronary heart disease (CHD), dilated cardiomyopathy (DCM), and valvular heart disease (VHD) remains poorly characterized in patients with HF from the view of metabolic profile. We sought to determine metabolic differences in CHD-, VHD-, and DCM-induced HF patients and identify significantly altered metabolites and their correlations. Procedure: In this study, a total of 96 HF cases and 97 controls were enrolled. The contents of 23 amino acids and 26 carnitines in fasting plasma were measured by a targeted liquid chromatography and mass spectrometry (LC-MS) approach. Results: Nine metabolites (Histidine, Arginine, Citrulline, Glutamine, Valine, hydroxyhexadecenyl-carnitine, acylcarnitine C22, hydroxytetradecanoyl-carnitine, and carnitine) were found to be related with the occurrence of HF. Arginine, Glutamine and hydroxytetradecanoyl-carnitine could effectively distinguish CHD and DCM patients, and hydroxytetradecanoyl-carnitine and aspartic acid were able to classify CHD and VHD cohorts. Conclusion: This study indicated that circulating amino acids and long-chain acylcarnitine levels were closely associated with progression of heart failure. Monitoring these metabolic alterations by LC-MS may help the differentiation of CHD, VHD, and DCM in the early stage, and provide new diagnostics targets or therapeutic interventions.
Purpose: Metabolic impairment is one key contributor to heart failure (HF) pathogenesis and progression. The major causes of HF, coronary heart disease (CHD), dilated cardiomyopathy (DCM), and valvular heart disease (VHD) remains poorly characterized in patients with HF from the view of metabolic profile. We sought to determine metabolic differences in CHD-, VHD-, and DCM-induced HF patients and identify significantly altered metabolites and their correlations. Procedure: In this study, a total of 96 HF cases and 97 controls were enrolled. The contents of 23 amino acids and 26 carnitines in fasting plasma were measured by a targeted liquid chromatography and mass spectrometry (LC-MS) approach. Results: Nine metabolites (Histidine, Arginine, Citrulline, Glutamine, Valine, hydroxyhexadecenyl-carnitine, acylcarnitineC22, hydroxytetradecanoyl-carnitine, and carnitine) were found to be related with the occurrence of HF. Arginine, Glutamine and hydroxytetradecanoyl-carnitine could effectively distinguish CHD and DCM patients, and hydroxytetradecanoyl-carnitine and aspartic acid were able to classify CHD and VHD cohorts. Conclusion: This study indicated that circulating amino acids and long-chain acylcarnitine levels were closely associated with progression of heart failure. Monitoring these metabolic alterations by LC-MS may help the differentiation of CHD, VHD, and DCM in the early stage, and provide new diagnostics targets or therapeutic interventions.
Authors: Svati H Shah; James R Bain; Michael J Muehlbauer; Robert D Stevens; David R Crosslin; Carol Haynes; Jennifer Dungan; L Kristin Newby; Elizabeth R Hauser; Geoffrey S Ginsburg; Christopher B Newgard; William E Kraus Journal: Circ Cardiovasc Genet Date: 2010-02-19
Authors: Tariq Ahmad; Jacob P Kelly; Robert W McGarrah; Anne S Hellkamp; Mona Fiuzat; Jeffrey M Testani; Teresa S Wang; Amanda Verma; Marc D Samsky; Mark P Donahue; Olga R Ilkayeva; Dawn E Bowles; Chetan B Patel; Carmelo A Milano; Joseph G Rogers; G Michael Felker; Christopher M O'Connor; Svati H Shah; William E Kraus Journal: J Am Coll Cardiol Date: 2016-01-26 Impact factor: 24.094