Literature DB >> 33551837

Chronic Cranial Windows for Long Term Multimodal Neurovascular Imaging in Mice.

Kıvılcım Kılıç1, Michèle Desjardins2, Jianbo Tang1,3, Martin Thunemann1, Smrithi Sunil1, Şefik Evren Erdener1,4, Dmitry D Postnov1,5, David A Boas1, Anna Devor1.   

Abstract

Chronic cranial windows allow for longitudinal brain imaging experiments in awake, behaving mice. Different imaging technologies have their unique advantages and combining multiple imaging modalities offers measurements of a wide spectrum of neuronal, glial, vascular, and metabolic parameters needed for comprehensive investigation of physiological and pathophysiological mechanisms. Here, we detail a suite of surgical techniques for installation of different cranial windows targeted for specific imaging technologies and their combination. Following these techniques and practices will yield higher experimental success and reproducibility of results.
Copyright © 2021 Kılıç, Desjardins, Tang, Thunemann, Sunil, Erdener, Postnov, Boas and Devor.

Entities:  

Keywords:  awake; chronic; imaging; mice; multimodal; neural; vascular

Year:  2021        PMID: 33551837      PMCID: PMC7862556          DOI: 10.3389/fphys.2020.612678

Source DB:  PubMed          Journal:  Front Physiol        ISSN: 1664-042X            Impact factor:   4.566


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