Negin Chavoshi Nezhad1, Zakaria Vahabzadeh2, Azra Allahveisie3, Khaled Rahmani4, Amir Raoofi5, Mohammad Jafar Rezaie6, Masoumeh Rezaei7, Maria Partovyan1. 1. MSc of Anatomical Sciences, Department of Anatomical Sciences, School of Medicine, Kurdistan University of Medical Sciences, Kurdistan, Iran. 2. Assistant Professor of Clinical Biochemistry, Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Kurdistan, Iran. 3. Assistant Professor of Reproductive Biology, Fertility and Infertility Research Center, Besat Medical Education and Treatment Center, Kurdistan University of Medical Sciences, Kurdistan, Iran. 4. Assistant Professor of Epidemiology, Liver and Digestive Research Center, Research Institute for Health Development Kurdistan University of Medical Sciences, Kurdistan, Iran. 5. Leishmaniosis Research Center, Department of Anatomy, Sabzevar University of Medical Sciences, Sabzevar, Iran. 6. Associate Professor of Anatomical Sciences, Fertility and Infertility Research Center, Besat Medical Education and Treatment Center, Kurdistan University of Medical Sciences, Kurdistan, Iran. Rezaiemjafar@gmail.com. 7. Assistant Professor of Obstetrics and Gynecology, Fertility and Infertility Research Center, Besat Medical Education and Treatment Center, Kurdistan University of Medical Sciences, Kurdistan, Iran.
Abstract
PURPOSE: The aim of the present study is to assess the effect of L-carnitine and Coenzyme Q10 (CoQ10) on human sperm motility, DNA fragmentation, chromatin structure, and reactive oxygen species (ROS) during, before and after freezing in oligospermia men. MATERIALS AND METHODS: Semen was collected from 30 oligospermic men, who referred to infertility clinic of Beasat Hospital in Sanandaj, Iran. The samples of each individual were divided into 8 equal parts: 1. control group before freezing; 2. incubated with L-carnitine; 3. incubated with coenzyme Q10; 4. incubated with the combination of L-carnitine + CoQ10; 5. control freezing group; 6. the experimental freezing group with L-carnitine; 7. the experimental freezing group with coenzyme Q10 and 8. the experimental freezing with the combination of L-c + CoQ10. Sperm motility was assessed by WET MOUNT method. DNA fragmentation was evaluated by SCD (Sperm Chromatin Desperation), ROS, was evaluated by quantitative fluorescence reaction, and chromatin deficiency was determined by chromatin staining (CMA3). RESULTS: Antioxidant treatments, significantly reduced the number of ROS + in the pre and post freezing groups. Significant improvement was seen in the sperm motility of class B in the pre freezing groups with L-carnitine. Antioxidants also reduced the percentage of DNA fragmentation and protamine deficiency in pre-and post-freezing. CONCLUSION: Addition of Coq10 and L-carnitine to human sperm medium significantly reduced the number of ROS. This reduction in ROS reduced sperm damage during cryopreservation.
PURPOSE: The aim of the present study is to assess the effect of L-carnitine and Coenzyme Q10 (CoQ10) on human sperm motility, DNA fragmentation, chromatin structure, and reactive oxygen species (ROS) during, before and after freezing in oligospermia men. MATERIALS AND METHODS: Semen was collected from 30 oligospermic men, who referred to infertility clinic of Beasat Hospital in Sanandaj, Iran. The samples of each individual were divided into 8 equal parts: 1. control group before freezing; 2. incubated with L-carnitine; 3. incubated with coenzyme Q10; 4. incubated with the combination of L-carnitine + CoQ10; 5. control freezing group; 6. the experimental freezing group with L-carnitine; 7. the experimental freezing group with coenzyme Q10 and 8. the experimental freezing with the combination of L-c + CoQ10. Sperm motility was assessed by WET MOUNT method. DNA fragmentation was evaluated by SCD (Sperm Chromatin Desperation), ROS, was evaluated by quantitative fluorescence reaction, and chromatin deficiency was determined by chromatin staining (CMA3). RESULTS: Antioxidant treatments, significantly reduced the number of ROS + in the pre and post freezing groups. Significant improvement was seen in the sperm motility of class B in the pre freezing groups with L-carnitine. Antioxidants also reduced the percentage of DNA fragmentation and protamine deficiency in pre-and post-freezing. CONCLUSION: Addition of Coq10 and L-carnitine to human sperm medium significantly reduced the number of ROS. This reduction in ROS reduced sperm damage during cryopreservation.