Xiaoyue Yu1,2, Yu Pan1,3, Yan Dong1,2, Bin Lu4, Chao Zhang1,2, Minsheng Yang5,6, Lihui Zuo7,8. 1. Forest Department, Forestry College, Hebei Agricultural University, Baoding, China. 2. Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, 071000, Baoding, P. R. China. 3. Tianjin nuohe medical laboratory co. LTD, Tianjin, China. 4. College of Landscape Architecture and Tourism, Hebei Agricultural University, Baoding, China. 5. Forest Department, Forestry College, Hebei Agricultural University, Baoding, China. yangms100@126.com. 6. Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, 071000, Baoding, P. R. China. yangms100@126.com. 7. Forest Department, Forestry College, Hebei Agricultural University, Baoding, China. zuolihui001@163.com. 8. College of Landscape and Ecological Engineering, Hebei University of Engineering, 056000, Handan, P. R. China. zuolihui001@163.com.
Abstract
BACKGROUND: As important forest tree species, biological stress and soil salinization are important factors that restrict the growth of Populus × euramericana. WRKYs are important transcription factors in plants that can regulate plant responses to biotic and abiotic stresses. In this study, PeWRKY31 was isolated from Populus × euramericana, and its bioinformation, salt resistance and insect resistance were analyzed. This study aims to provide guidance for producing salt-resistant and insect-resistant poplars. RESULTS: PeWRKY31 has a predicted open reading frame (ORF) of 1842 bp that encodes 613 amino acids. The predicted protein is the unstable, acidic, and hydrophilic protein with a molecular weight of 66.34 kDa, and it has numerous potential phosphorylation sites, chiefly on serines and threonines. PeWRKY31 is a zinc-finger C2H2 type-II WRKY TF that is closely related to WRKY TFs of Populus tomentosa, and localizes to the nucleus. A PeWRKY31 overexpression vector was constructed and transformed into Nicotiana tabacum L. Overexpression of PeWRKY31 improved the salt tolerance and insect resistance of the transgenic tobacco. Transcriptome sequencing and KEGG enrichment analysis showed the elevated expression of genes related to glutathione metabolism, plant hormone signal transduction, and MAPK signaling pathways, the functions of which were important in plant salt tolerance and insect resistance in the overexpressing tobacco line. CONCLUSIONS: PeWRKY31 was isolated from Populus × euramericana. Overexpression of PeWRKY31 improved the resistance of transgenic plant to salt stress and pest stress. The study provides references for the generation of stress-resistant lines with potentially great economic benefit.
BACKGROUND: As important forest tree species, biological stress and soil salinization are important factors that restrict the growth of Populus × euramericana. WRKYs are important transcription factors in plants that can regulate plant responses to biotic and abiotic stresses. In this study, PeWRKY31 was isolated from Populus × euramericana, and its bioinformation, salt resistance and insect resistance were analyzed. This study aims to provide guidance for producing salt-resistant and insect-resistant poplars. RESULTS: PeWRKY31 has a predicted open reading frame (ORF) of 1842 bp that encodes 613 amino acids. The predicted protein is the unstable, acidic, and hydrophilic protein with a molecular weight of 66.34 kDa, and it has numerous potential phosphorylation sites, chiefly on serines and threonines. PeWRKY31 is a zinc-finger C2H2 type-II WRKY TF that is closely related to WRKY TFs of Populus tomentosa, and localizes to the nucleus. A PeWRKY31 overexpression vector was constructed and transformed into Nicotiana tabacum L. Overexpression of PeWRKY31 improved the salt tolerance and insect resistance of the transgenic tobacco. Transcriptome sequencing and KEGG enrichment analysis showed the elevated expression of genes related to glutathione metabolism, plant hormone signal transduction, and MAPK signaling pathways, the functions of which were important in plant salt tolerance and insect resistance in the overexpressing tobacco line. CONCLUSIONS: PeWRKY31 was isolated from Populus × euramericana. Overexpression of PeWRKY31 improved the resistance of transgenic plant to saltstress and pest stress. The study provides references for the generation of stress-resistant lines with potentially great economic benefit.
Entities:
Keywords:
Genetic transformation; Insect resistance; Salt tolerance; Transcriptome; WRKY