Understanding ventilation and oxygen uptake of Pacific hagfish (Eptatretus stoutii), with particular emphasis on responses to ammonia and interactions with other respiratory gases.

The hagfishes are an ancient and evolutionarily important group, with breathing mechanisms and gills very different from those of other fishes. Hagfish inhale through a single nostril via a velum pump, and exhale through multiple separate gill pouches. We assessed respiratory performance in E. stoutii (31 ppt, 12 ºC, 50-120 g) by measuring total ventilatory flow ([Formula: see text]) at the nostril, velar (respiratory) frequency (fr), and inspired (PIO2) and expired (PEO2) oxygen tensions at all 12 gill pouch exits plus the pharyngo-cutaneous duct (PCD) on the left side, and calculated ventilatory stroke volume (S[Formula: see text]), % O2 utilization, and oxygen consumption (ṀO2). At rest under normoxia, spontaneous changes in [Formula: see text] ranged from apnea to > 400 ml kg-1 min-1, due to variations in both fr and S[Formula: see text]; "normal" [Formula: see text] averaged 137 ml kg-1 min-1, ṀO2 was 718 µmol kg-1 h-1, so the ventilatory convection requirement for O2 was about 11 L mmol-1. Relative to anterior gill pouches, lower PEO2 values (i.e. higher utilization) occurred in the more posterior pouches and PCD. Overall, O2 utilization was 34% and did not change during hyperventilation but increased to > 90% during hypoventilation. Environmental hypoxia (PIO2 ~ 8% air saturation, 1.67 kPa, 13 Torr) caused hyperventilation, but neither acute hyperoxia (PIO2 ~ 275% air saturation, 57.6 kPa, 430 Torr) nor hypercapnia (PICO2 ~ 1% CO2, 1.0 kPa, 7.5 Torr) significantly altered [Formula: see text]. ṀO2 decreased in hypoxia and increased in hyperoxia but did not change in hypercapnia. Acute exposure to high environmental ammonia (HEA, 10 mM NH4HCO3) caused an acute decrease in [Formula: see text], in contrast to the hyperventilation of long-term HEA exposure described in a previous study. The hypoventilatory response to HEA still occurred during hypoxia and hyperoxia, but was blunted during hypercapnia. Under all treatments, ṀO2 increased with increases in [Formula: see text]. Overall, there were lower convection requirements for O2 during hyperoxia, higher requirements during hypoxia and hypercapnia, but unchanged requirements during HEA. We conclude that this "primitive" fish operates a flexible respiratory system with considerable reserve capacity.