June Seok Heo1, Sinyoung Kim2, Chae Eun Yang3, Youjeong Choi1, Seung Yong Song4, Hyun Ok Kim5,6. 1. Cell Therapy Center, Severance Hospital, Seoul, 03722, Republic of Korea. 2. Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea. 3. Department of Plastic Surgery, Severance Christian Hospital, Wonju, 26426, Republic of Korea. 4. Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea. PSSYSONG@yuhs.ac. 5. Cell Therapy Center, Severance Hospital, Seoul, 03722, Republic of Korea. HYUNOK1019@yuhs.ac. 6. Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea. HYUNOK1019@yuhs.ac.
Abstract
BACKGROUND: Human adipose-derived mesenchymal stem cells (AMSCs) are an attractive resource for wound healing because their regenerative capacity improves injury repair. Recently, stem cell-derived exosomes have been shown to play a positive role in stem cell-based therapies. However, the effects of exosomes derived from AMSCs (AEXOs) on wound healing are unclear. In this study, we aimed to examine the role of AEXOs in attenuating inflammation and explore their effects in normal wound healing. METHODS: We isolated exosomes from AMSCs and established a cellular model of inflammation by treatment with the inflammatory cytokines, interferon gamma and tumor necrosis factor alpha, to determine whether AEXOs can inhibit inflammation. We examined the wound healing effects of AEXOs in in vitro wound healing models and performed a miRNA array to understand the role of AEXOs in inflammation and wound healing. RESULTS: A significant difference was observed in wound closure and the expression of anti-inflammatory and wound-healing-related factors between control and AEXO-treated cells. CONCLUSION: Our results showed that besides alleviating the inflammation response, AEXOs also promote wound healing. Thus, AEXOs represent a novel, stem-cell-based, therapeutic strategy for wound healing.
BACKGROUND: Human adipose-derived mesenchymal stem cells (AMSCs) are an attractive resource for wound healing because their regenerative capacity improves injury repair. Recently, stem cell-derived exosomes have been shown to play a positive role in stem cell-based therapies. However, the effects of exosomes derived from AMSCs (AEXOs) on wound healing are unclear. In this study, we aimed to examine the role of AEXOs in attenuating inflammation and explore their effects in normal wound healing. METHODS: We isolated exosomes from AMSCs and established a cellular model of inflammation by treatment with the inflammatory cytokines, interferon gamma and tumor necrosis factor alpha, to determine whether AEXOs can inhibit inflammation. We examined the wound healing effects of AEXOs in in vitro wound healing models and performed a miRNA array to understand the role of AEXOs in inflammation and wound healing. RESULTS: A significant difference was observed in wound closure and the expression of anti-inflammatory and wound-healing-related factors between control and AEXO-treated cells. CONCLUSION: Our results showed that besides alleviating the inflammation response, AEXOs also promote wound healing. Thus, AEXOs represent a novel, stem-cell-based, therapeutic strategy for wound healing.
Authors: Mohamed Gadelkarim; Abdelrahman Ibrahim Abushouk; Esraa Ghanem; Ali Mohamed Hamaad; Anas M Saad; Mohamed M Abdel-Daim Journal: Biomed Pharmacother Date: 2018-08-14 Impact factor: 6.529