Reihaneh Haghniaz1,2, Atiya Rabbani3, Fereshteh Vajhadin1,4, Taous Khan5, Rozina Kousar6, Abdul Rehman Khan3, Hossein Montazerian1, Javed Iqbal7, Alberto Libanori1, Han-Jun Kim8, Fazli Wahid9. 1. Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA. 2. California NanoSystems Institute (CNSI), University of California, Los Angeles, Los Angeles, CA, 90095, USA. 3. Department of Biotechnology, COMSATS University Islamabad, Islamabad, 45550, Pakistan. 4. Department of Chemistry, Yazd University, 89195-741, Yazd, Iran. 5. Department of Pharmacy, COMSATS University Islamabad, Islamabad, 45550, Pakistan. 6. Department of Pharmacy, Women Institute of Learning, Abbottabad, 22060, Pakistan. 7. Department of Botany, Bacha Khan University, Charsadda, 24420, Pakistan. 8. Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064, USA. hkim@terasaki.org. 9. Department of Biomedical Sciences, Pak-Austria Fachhochschule: Institute of Applied Sciences and Technology, Haripur, 22620, Pakistan. fazli.wahid@fbse.paf-iast.edu.pk.
Abstract
BACKGROUND: Increasing antibiotic resistance continues to focus on research into the discovery of novel antimicrobial agents. Due to its antimicrobial and wound healing-promoting activity, metal nanoparticles have attracted attention for dermatological applications. This study is designed to investigate the scope and bactericidal potential of zinc ferrite nanoparticles (ZnFe2O4 NPs), and the mechanism of anti-bacterial action along with cytocompatibility, hemocompatibility, and wound healing properties. RESULTS: ZnFe2O4 NPs were synthesized via a modified co-precipitation method. Structure, size, morphology, and elemental compositions of ZnFe2O4 NPs were analyzed using X-ray diffraction pattern, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy. In PrestoBlue and live/dead assays, ZnFe2O4 NPs exhibited dose-dependent cytotoxic effects on human dermal fibroblasts. In addition, the hemocompatibility assay revealed that the NPs do not significantly rupture red blood cells up to a dose of 1000 µg/mL. Bacterial live/dead imaging and zone of inhibition analysis demonstrated that ZnFe2O4 NPs showed dose-dependent bactericidal activities in various strains of Gram-negative and Gram-positive bacteria. Interestingly, NPs showed antimicrobial activity through multiple mechanisms, such as cell membrane damage, protein leakage, and reactive oxygen species generation, and were more effective against gram-positive bacteria. Furthermore, in vitro scratch assay revealed that ZnFe2O4 NPs improved cell migration and proliferation of cells, with noticeable shrinkage of the artificial wound model. CONCLUSIONS: This study indicated that ZnFe2O4 NPs have the potential to be used as a future antimicrobial and wound healing drug.
BACKGROUND: Increasing antibiotic resistance continues to focus on research into the discovery of novel antimicrobial agents. Due to its antimicrobial and wound healing-promoting activity, metal nanoparticles have attracted attention for dermatological applications. This study is designed to investigate the scope and bactericidal potential of zinc ferrite nanoparticles (ZnFe2O4 NPs), and the mechanism of anti-bacterial action along with cytocompatibility, hemocompatibility, and wound healing properties. RESULTS:ZnFe2O4 NPs were synthesized via a modified co-precipitation method. Structure, size, morphology, and elemental compositions of ZnFe2O4 NPs were analyzed using X-ray diffraction pattern, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy. In PrestoBlue and live/dead assays, ZnFe2O4 NPs exhibited dose-dependent cytotoxic effects on human dermal fibroblasts. In addition, the hemocompatibility assay revealed that the NPs do not significantly rupture red blood cells up to a dose of 1000 µg/mL. Bacterial live/dead imaging and zone of inhibition analysis demonstrated that ZnFe2O4 NPs showed dose-dependent bactericidal activities in various strains of Gram-negative and Gram-positive bacteria. Interestingly, NPs showed antimicrobial activity through multiple mechanisms, such as cell membrane damage, protein leakage, and reactive oxygen species generation, and were more effective against gram-positive bacteria. Furthermore, in vitro scratch assay revealed that ZnFe2O4 NPs improved cell migration and proliferation of cells, with noticeable shrinkage of the artificial wound model. CONCLUSIONS: This study indicated that ZnFe2O4 NPs have the potential to be used as a future antimicrobial and wound healing drug.
Authors: Jessica M A Blair; Mark A Webber; Alison J Baylay; David O Ogbolu; Laura J V Piddock Journal: Nat Rev Microbiol Date: 2014-12-01 Impact factor: 60.633
Authors: Rania Dadi; Rabah Azouani; Mamadou Traore; Christine Mielcarek; Andrei Kanaev Journal: Mater Sci Eng C Mater Biol Appl Date: 2019-07-09 Impact factor: 7.328