Jing Zheng1, Tian-Ke Li, Yang Bao, Su-Xin Zhang. 1. Department of Stomatology, The Fourth Hospital of Hebei Medical University.Shijiazhuang 050011, Hebei Province, China. E-mail:258481978@qq.com.
Abstract
PURPOSE: To investigate the expression and significance of Spindly and Bub3 in oral squamous cell carcinoma(OSCC). METHODS: Sixty-five patients with OSCC admitted to the Fourth Hospital of Hebei Medical University from March 2017 to March 2019 were enrolled. RT-PCR was used to detect the expression of Spindly and Bub3 mRNA in oral squamous cell carcinoma and adjacent normal tissues from the patients. OSCC cell line was cultured. After siRNA transfection interference with the expression of Spindly and Bub3 genes, cell viability was detected by MTT assay, and the cell migration ability was detected by scratch test. Statistical analysis was performed with SPSS 22.0 software package RESULTS: The expression levels of Spindly and Bub3 mRNA in OSCC were significantly higher in adjacent tissues(P<0.05). The expression of Spindly and Bub3 mRNA was related to TNM staging, clinical staging and lymph node metastasis (P<0.05). The 5-year survival rate of patients with high expression of Spindly, Bub3, Spindly/Bub3 were significantly higher than the counterparts with low expression, and the 5-year survival rate of patients with high expression of Spindly/Bub3 was significantly lower than that of patients with high expression of Spindly and Bub3(P<0.05). The expression level of Spindly in siRNA-Spindly group was significantly lower than that in Spindly negative control group and blank control group, and the expression level of Bub3 in siRNA-Bub3 group was also significantly lower than that in Bub3 negative control group and blank control group. The expression level of Spindly in siRNA-Spindly group was significantly lower than that in Spindly negative control group and blank control group, and the expression level of Bub3 in siRNA-Bub3 group was also lower than that in Bub3 negative control group and blank control group. The migration ability of cells in siRNA-Spindly group at 24 and 48 hours was significantly lower than that in Spindly negative control group and blank control group; the migration ability at 24 and 48 hours was significantly lower than that of Bub3 negative control group and blank control group(P<0.05). CONCLUSIONS: Spindly and Bub3 are highly expressed in OSCC. Specific inhibition of Spindly and Bub3 gene expression can reduce the proliferation and migration of cancer cells, which might be used as one of the targets for the treatment of OSCC.
PURPOSE: To investigate the expression and significance of Spindly and Bub3 in oral squamous cell carcinoma(OSCC). METHODS: Sixty-five patients with OSCC admitted to the Fourth Hospital of Hebei Medical University from March 2017 to March 2019 were enrolled. RT-PCR was used to detect the expression of Spindly and Bub3 mRNA in oral squamous cell carcinoma and adjacent normal tissues from the patients. OSCC cell line was cultured. After siRNA transfection interference with the expression of Spindly and Bub3 genes, cell viability was detected by MTT assay, and the cell migration ability was detected by scratch test. Statistical analysis was performed with SPSS 22.0 software package RESULTS: The expression levels of Spindly and Bub3 mRNA in OSCC were significantly higher in adjacent tissues(P<0.05). The expression of Spindly and Bub3 mRNA was related to TNM staging, clinical staging and lymph node metastasis (P<0.05). The 5-year survival rate of patients with high expression of Spindly, Bub3, Spindly/Bub3 were significantly higher than the counterparts with low expression, and the 5-year survival rate of patients with high expression of Spindly/Bub3 was significantly lower than that of patients with high expression of Spindly and Bub3(P<0.05). The expression level of Spindly in siRNA-Spindly group was significantly lower than that in Spindly negative control group and blank control group, and the expression level of Bub3 in siRNA-Bub3 group was also significantly lower than that in Bub3 negative control group and blank control group. The expression level of Spindly in siRNA-Spindly group was significantly lower than that in Spindly negative control group and blank control group, and the expression level of Bub3 in siRNA-Bub3 group was also lower than that in Bub3 negative control group and blank control group. The migration ability of cells in siRNA-Spindly group at 24 and 48 hours was significantly lower than that in Spindly negative control group and blank control group; the migration ability at 24 and 48 hours was significantly lower than that of Bub3 negative control group and blank control group(P<0.05). CONCLUSIONS: Spindly and Bub3 are highly expressed in OSCC. Specific inhibition of Spindly and Bub3 gene expression can reduce the proliferation and migration of cancer cells, which might be used as one of the targets for the treatment of OSCC.