Investigation of effect of ultrasound on immobilized C. rugosa lipase: Synthesis of biomass based furfuryl derivative and green metrics evaluation study.

The present work deals with the synthesis of lab-made carboxymethyl cellulose (CMC) and chitosan (CHI) based co-polymer cross-linked with glutaraldehyde (GLU) which is used as immobilization matrix for the immobilization of Candida rugosa (CRL) lipase (CMC:CHI:GLU:CRL). This immobilized biocatalyst was subjected to characterization such as lipase-activity, kinetic-parameters, water-content, surface-texture, stability and half-life time etc. Effect of various ultrasound parameters (power, frequency, duty cycle, exposure time) on lipase activity is also tested which indicated that, developed biocatalyst has significant activity-stability and half-life-time in ultrasonicated medium. Further, this biocatalyst was applied to synthesize biomass-derived furfuryl derivative which offering excellent conversion of 99 % of bio-based furfuryl ester. The synthetic protocol is optimized in detail (with twelve reaction parameters) under ultrasonicated medium. Recyclability study offered 68 % conversion of the furfuryl ester after sixth reuse. Moreover, the developed protocol is well extended to synthesize various commercially important compounds. Besides this, we investigated thermodynamic parameters (ΔG*, ΔH*, ΔS*) which demonstrating more feasibility of biocatalytic synthesis in ultrasonicated medium than conventional medium. Finally, green metrics evaluation parameters (E-factor, carbon-efficiency and mass-intensity) are studied which indicating efficient synergetic role of immobilized CMC:CHI:GLU:CRL lipase biocatalysis and ultrasonication in green and sustainable synthesis.