| Literature DB >> 33539396 |
Shinichi Hashimoto1,2, Takayoshi Shirasaki3, Taro Yamashita3, Sadahiro Iwabuchi1,2, Yutaka Suzuki4, Yuzuru Takamura5, Yoshiaki Ukita6, Shungo Deshimaru7, Toshitugu Okayama8, Kazuho Ikeo8, Kazuyuki Kuroki3, Kazunori Kawaguchi3, Eishiro Mizukoshi3, Kouji Matsushima7, Masao Honda3, Shuichi Kaneko3.
Abstract
Human hepatitis B virus (HBV) infection remains a serious health problem worldwide. However, the mechanism for the maintenance of HBV in a latent state within host cells remains unclear. Here, using single-cell RNA sequencing analysis, we identified four genes linked to the maintenance of HBV in a liver cell line expressing HBV RNA at a low frequency. These genes included DOCK11 and DENND2A, which encode small GTPase regulators. In primary human hepatocytes infected with HBV, knockdown of these two genes decreased the amount of both HBV DNA and covalently closed circular DNA to below the limit of detection. Our findings reveal a role for DOCK11 and DENND2A in the maintenance of HBV.Entities:
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Year: 2021 PMID： 33539396 PMCID： PMC7861363 DOI： 10.1371/journal.pone.0246313
Source DB: PubMed Journal: PLoS One ISSN： 1932-6203 Impact factor: 3.240