Carlos van der Putten1,2, Ester B M Remmerswaal1,2, Matty L Terpstra1,2, Nelly D van der Bom1,2, Jesper Kers3, Ineke J M Ten Berge1,2, Suzanne E Geerlings4, René A W van Lier5, Frederike J Bemelman1,2, Michiel C van Aalderen1,2. 1. Department of Experimental Immunology, Amsterdam institute for Infection & Immunity, Amsterdam UMC, University of Amsterdam, 1105AZ Amsterdam, The Netherlands. 2. Division of Internal Medicine, Department of Nephrology, Renal Transplant Unit, Amsterdam Infection & Immunity Institute (AI&II), Amsterdam UMC, University of Amsterdam, 1105AZ Amsterdam, The Netherlands. 3. Department of Pathology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands. 4. Department of Internal Medicine, Infectious Diseases, Amsterdam Infection & Immunity Institute (AI&II), Amsterdam UMC, University of Amsterdam, 1105AZ Amsterdam, The Netherlands. 5. Sanquin Research and Landsteiner laboratory, 1066CX Amsterdam, The Netherlands.
Abstract
BACKGROUND: At border sites, and in internal organs, tissue resident memory T cells (TRM) contribute to the immune barrier against pathogens like viruses, bacteria, fungi, and cancer. However, information on the presence and function of these cells in the human kidney is scant. In order to better understand the T cell-mediated immunological defense in this organ, we aimed to determine phenotypic and functional aspects of CD8 and CD4 T cells present in healthy and allograft kidney tissue. METHODS: Using multichannel flow cytometry, we assessed the phenotype and function of T cells in healthy renal tissue samples (n = 5) and kidney allograft tissue (n = 7) and compared these aspects to T cells in peripheral blood from healthy controls (n = 13). RESULTS: Kidney tissue samples contained substantial amounts of CD8 and CD4 T cells. In contrast to the circulating cells, kidney T cells frequently expressed CD69 and CD103, and were more often actively cycling. Furthermore, nearly all kidney T cells expressed CXCR3, and often expressed CXCR6 compared to T cells in the circulation. Markedly, kidney T cells produced greater quantities of IFNγ than circulating cells and were frequently polyfunctional. CONCLUSION: Functional T cells with the characteristic traits of TRM reside in human kidney tissues. These cells are more often actively cycling and frequently express CXCR3 and CXCR6.
BACKGROUND: At border sites, and in internal organs, tissue resident memory T cells (TRM) contribute to the immune barrier against pathogens like viruses, bacteria, fungi, and cancer. However, information on the presence and function of these cells in the human kidney is scant. In order to better understand the T cell-mediated immunological defense in this organ, we aimed to determine phenotypic and functional aspects of CD8 and CD4 T cells present in healthy and allograft kidney tissue. METHODS: Using multichannel flow cytometry, we assessed the phenotype and function of T cells in healthy renal tissue samples (n = 5) and kidney allograft tissue (n = 7) and compared these aspects to T cells in peripheral blood from healthy controls (n = 13). RESULTS: Kidney tissue samples contained substantial amounts of CD8 and CD4 T cells. In contrast to the circulating cells, kidney T cells frequently expressed CD69 and CD103, and were more often actively cycling. Furthermore, nearly all kidney T cells expressed CXCR3, and often expressed CXCR6 compared to T cells in the circulation. Markedly, kidney T cells produced greater quantities of IFNγ than circulating cells and were frequently polyfunctional. CONCLUSION: Functional T cells with the characteristic traits of TRM reside in human kidney tissues. These cells are more often actively cycling and frequently express CXCR3 and CXCR6.
Authors: Ester B M Remmerswaal; Simone H C Havenith; Mirza M Idu; Ester M M van Leeuwen; Karlijn A M I van Donselaar; Anja Ten Brinke; Nelly van der Bom-Baylon; Fréderike J Bemelman; René A W van Lier; Ineke J M Ten Berge Journal: Blood Date: 2011-12-29 Impact factor: 22.113
Authors: Pleun Hombrink; Christina Helbig; Ronald A Backer; Berber Piet; Anna E Oja; Regina Stark; Giso Brasser; Aldo Jongejan; René E Jonkers; Benjamin Nota; Onur Basak; Hans C Clevers; Perry D Moerland; Derk Amsen; René A W van Lier Journal: Nat Immunol Date: 2016-10-24 Impact factor: 25.606
Authors: Nina L Fransen; Cheng-Chih Hsiao; Marlijn van der Poel; Hendrik J Engelenburg; Kim Verdaasdonk; Maria C J Vincenten; Ester B M Remmerswaal; Tanja Kuhlmann; Matthew R J Mason; Jörg Hamann; Joost Smolders; Inge Huitinga Journal: Brain Date: 2020-06-01 Impact factor: 13.501
Authors: Sofia Caldeira-Dantas; Thomas Furmanak; Corinne Smith; Michael Quinn; Leyla Y Teos; Adam Ertel; Drishya Kurup; Mayank Tandon; Ilias Alevizos; Christopher M Snyder Journal: J Immunol Date: 2017-12-29 Impact factor: 5.422
Authors: Taheri Sathaliyawala; Masaru Kubota; Naomi Yudanin; Damian Turner; Philip Camp; Joseph J C Thome; Kara L Bickham; Harvey Lerner; Michael Goldstein; Megan Sykes; Tomoaki Kato; Donna L Farber Journal: Immunity Date: 2012-12-20 Impact factor: 31.745
Authors: Michiel C van Aalderen; Ester B M Remmerswaal; Kirstin M Heutinck; Anja Ten Brinke; Mariet C W Feltkamp; Neelke C van der Weerd; Karlijn A M I van der Pant; Frederike J Bemelman; René A W van Lier; Ineke J M Ten Berge Journal: PLoS Pathog Date: 2016-10-10 Impact factor: 6.823
Authors: Loreto Parga-Vidal; Michiel C van Aalderen; Regina Stark; Klaas P J M van Gisbergen Journal: Nat Rev Nephrol Date: 2022-01-25 Impact factor: 28.314
Authors: Lingyun Kong; Sofianos Andrikopoulos; Richard J MacIsaac; Laura K Mackay; David J Nikolic-Paterson; Niloufar Torkamani; Neda Zafari; Evelyn C S Marin; Elif I Ekinci Journal: J Diabetes Investig Date: 2021-12-21 Impact factor: 4.232
Authors: Jennifer C Whitesell; Robin S Lindsay; Jessica G Olivas-Corral; Seth F Yannacone; Mary H Schoenbach; Erin D Lucas; Rachel S Friedman Journal: Front Immunol Date: 2022-01-25 Impact factor: 7.561
Authors: Michiel G H Betjes; Frederique Prevoo; Thierry P P van den Bosch; Mariska Klepper; Nicolle H R Litjens Journal: Cells Date: 2022-07-18 Impact factor: 7.666