Giorgio Silva-Santana1,2,3, Juliet Cunha Bax4, Débora Cristina Silva Fernandes2,3, Daniela Tendler Leibel Bacellar5, Cleber Hooper6, Alexandre Alves Souza Oliveira Dias5, Cristina Barbosa Silva7, Aline Moreira de Souza4, Simone Ramos5, Ricardo Alexandre Santos5, Thainara Ramos Pinto5, Mariana Antunes Ramão5, Ana Luíza Mattos-Guaraldi1,2,3,8. 1. Health Sciences Center Institute of Microbiology Paulo de Góes Federal University of Rio de Janeiro Rio de Janeiro RJ Brazil. 2. Laboratory of Diphtheria and Corynebacteria of Clinical Relevance Faculty of Medical Sciences University of the State of Rio de Janeiro Rio de Janeiro RJ Brazil. 3. The Collaborating Centre for Reference and Research on Diphtheria/National Health Foundation/Ministry of Health Rio de Janeiro RJ Brazil. 4. Laboratory of Clinical Research and Molecular Diagnostic Prof. Marcílio Dias do Nascimento Department of Clinical Practice and Pathology Fluminense Federal University Niterói RJ Brazil. 5. Laboratory Bacterial Vaccines Department of Immunology National Institute for Quality Control in Health Oswaldo Cruz Foundation Rio de Janeiro RJ Brazil. 6. Institute of Science and Technology in Biomodels Oswaldo Cruz Foundation Rio de Janeiro RJ Brazil. 7. Institute of Biology Federal Fluminense University Niterói RJ Brazil. 8. Biomedical Center College of Medical Sciences University of the State of Rio de Janeiro Rio de Janeiro RJ Brazil.
Abstract
BACKGROUND: Animal models are widely used in scientific research in order to obtain information from a whole organism under a specific set of experimental conditions. Various lineages of mice have been used to investigate diseases and new therapeutic strategies, and, consequently, hematological and biochemical tests in these laboratory animals are essential to validate scientific studies. Our study seeks to establish reference values for hematological and biochemical parameters of four lineages of mice. METHODS: We evaluated the hematological and biochemical profiles of 20 males and 20 females from the lineages Swiss (heterogeneous), BALB/c and C57BL/6 (isogenic), and B6D2F1 (hybrid), totaling 160 mice. Analysis were standardized using the systems pocH-100iV Diff™ for 19 hematological parameters and VITROS® 350 for 12 biochemical parameters. RESULTS: Results are shown as means and standard deviation, grouped by lineage and genre. Comparing the values obtained in this study with the values from previous studies, some variations were detected, which could be explained by differences in methodologies or individual variability. CONCLUSION: Thus our study shows that knowledge and disclosure of the values of physiological parameters of laboratory animals is necessary, and emphasises the importance of considering variations influenced by gender, lineage and genotype in the choice of the best experimental model.
BACKGROUND: Animal models are widely used in scientific research in order to obtain information from a whole organism under a specific set of experimental conditions. Various lineages of mice have been used to investigate diseases and new therapeutic strategies, and, consequently, hematological and biochemical tests in these laboratory animals are essential to validate scientific studies. Our study seeks to establish reference values for hematological and biochemical parameters of four lineages of mice. METHODS: We evaluated the hematological and biochemical profiles of 20 males and 20 females from the lineages Swiss (heterogeneous), BALB/c and C57BL/6 (isogenic), and B6D2F1 (hybrid), totaling 160 mice. Analysis were standardized using the systems pocH-100iV Diff™ for 19 hematological parameters and VITROS® 350 for 12 biochemical parameters. RESULTS: Results are shown as means and standard deviation, grouped by lineage and genre. Comparing the values obtained in this study with the values from previous studies, some variations were detected, which could be explained by differences in methodologies or individual variability. CONCLUSION: Thus our study shows that knowledge and disclosure of the values of physiological parameters of laboratory animals is necessary, and emphasises the importance of considering variations influenced by gender, lineage and genotype in the choice of the best experimental model.
Authors: Gordon P Otto; Birgit Rathkolb; Manuela A Oestereicher; Christoph J Lengger; Corinna Moerth; Kateryna Micklich; Helmut Fuchs; Valérie Gailus-Durner; Eckhard Wolf; Martin Hrabě de Angelis Journal: J Am Assoc Lab Anim Sci Date: 2016 Impact factor: 1.232
Authors: Tatyana Adayev; Giuseppe LaFauci; Weimin Xu; Carl Dobkin; Richard Kascsak; W Ted Brown; Jeffrey H Goodman Journal: Genes (Basel) Date: 2021-09-26 Impact factor: 4.096