Literature DB >> 3353221

Regen: program for designing gene assembly.

R Jerala1, V Turk.   

Abstract

One of the main problems in constructing synthetic genes is the incorrect hybridisation between the oligonucleotides. The problem is resolved if the sequence uniquely defines the position of the oligonucleotide in the assembled gene. This can be accomplished through the wise partition of dsDNA sequence in the fragments. We describe a program for use in designing such gene assembly. For a given DNA sequence and the approximate location of oligonucleotide boundary it generates all sets of protruding ends that share the smallest homology.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3353221      PMCID： PMC338169          DOI： 10.1093/nar/16.5.1759

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  12 in total

1.  Chemical synthesis of a gene for somatomedin C.

Authors:  B S Sproat; M J Gait
Journal:  Nucleic Acids Res       Date:  1985-04-25       Impact factor: 16.971

2.  Predicting DNA duplex stability from the base sequence.

Authors:  K J Breslauer; R Frank; H Blöcker; L A Marky
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

3.  An accelerated algorithm for calculating the secondary structure of single stranded RNAs.

Authors:  E Comay; R Nussinov; O Comay
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

4.  An alternate method for synthesis of double-stranded DNA segments.

Authors:  J J Rossi; R Kierzek; T Huang; P A Walker; K Itakura
Journal:  J Biol Chem       Date:  1982-08-25       Impact factor: 5.157

5.  Pattern recognition in nucleic acid sequences. II. An efficient method for finding locally stable secondary structures.

Authors:  M I Kanehisa; W B Goad
Journal:  Nucleic Acids Res       Date:  1982-01-11       Impact factor: 16.971

6.  Enhanced graphic matrix analysis of nucleic acid and protein sequences.

Authors:  J V Maizel; R P Lenk
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

7.  Cloning and expression in Escherichia coli of a synthetic DNA for hirudin, the blood coagulation inhibitor in the leech.

Authors:  E Fortkamp; M Rieger; G Heisterberg-Moutses; S Schweitzer; R Sommer
Journal:  DNA       Date:  1986-12

8.  Protein inhibitors of cysteine proteinases. III. Amino-acid sequence of cystatin from chicken egg white.

Authors:  V Turk; J Brzin; M Longer; A Ritonja; M Eropkin; U Borchart; W Machleidt
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1983-11

Review 9.  Total synthesis of a gene.

Authors:  H G Khorana
Journal:  Science       Date:  1979-02-16       Impact factor: 47.728

10.  Total synthesis of a gene for bovine rhodopsin.

Authors:  L Ferretti; S S Karnik; H G Khorana; M Nassal; D D Oprian
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.