Ya Wu1,2,3,4, Xueya Li5, Fang Tan6, Xianrong Zhou1,2,3, Jianfei Mu1,2,3, Xin Zhao7,8,9. 1. Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan'an District, Chongqing, 400067, People's Republic of China. 2. Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan'an District, Chongqing, 400067, People's Republic of China. 3. Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan'an District, Chongqing, 400067, People's Republic of China. 4. College of Biological and Chemical Engineering, Chongqing University of Education, Xuefu Main Street 9 Nan'an District, Chongqing, 400067, China. 5. Department of Dermatology, People's Hospital of Chongqing Banan District, 659 Yunan Avenue, Longzhouwan Street, Banan District, Chongqing, 401320, China. 6. Department of Public Health, Our Lady of Fatima University, 838, Valenzuela, Philippines. 7. Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan'an District, Chongqing, 400067, People's Republic of China. zhaoxin@cque.edu.cn. 8. Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan'an District, Chongqing, 400067, People's Republic of China. zhaoxin@cque.edu.cn. 9. Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan'an District, Chongqing, 400067, People's Republic of China. zhaoxin@cque.edu.cn.
Abstract
BACKGROUND: Obesity is an epidemic disease in the world, the treatment and prevention of obesity methods have gained great attention. Lactobacillus is the main member of probiotics, and the physiological activity of it is specific to different strains. This study systematically explored the anti-obesity effect and possible mechanism of Lactobacillus fermentum CQPC07 (LF-CQPC07), which was isolated from pickled vegetables. RESULTS: LF-CQPC07 effectively controlled the weight gain of mice caused by a high-fat diet. The results of pathological sections indicated that LF-CQPC07 alleviated hepatocyte damage and fat accumulation in adipocytes. The detection of biochemical indictors revealed that LF-CQPC07 decreased the levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG), and increased the level of high-density lipoprotein cholesterol (HDL-C). Additionally, LF-CQPC07 caused the decrease in the amounts of inflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-α (TNF-α), IL-6, and interferon-γ (IFN-γ), and the increase in the amounts of the anti-inflammatory cytokines IL-10 and IL-4. LF-CQPC07 also decreased the amounts of alanine aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP). Confirmed by qPCR, LF-CQPC07 enhanced the mRNA expression of catalase (CAT), gamma glutamylcysteine synthetase 1 (GSH1), copper/zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), and glutathione peroxidase (GSH-Px). It also increased the mRNA expression levels of carnitine palmitoyltransferase 1 (CPT1), peroxisome proliferator-activated receptor alpha (PPAR-α), lipoprotein lipase (LPL), and cholesterol 7 alpha hydroxylase (CYP7A1), and decreased that of PPAR-γ and CCAAT/enhancer binding protein alpha (C/EBP-α) in the liver of mice. CONCLUSION: This research confirmed that LF-CQPC07 is capable of ameliorating obesity, improving hyperlipemia, and alleviating chronic low-grade inflammation and liver injury accompanied with obesity. Its mechanism may be the regulation of antioxidant capacity and lipid metabolism. Therefore, LF-CQPC07 has enormous potential to serve as a potential probiotic for the prevention or treatment of obesity.
BACKGROUND:Obesity is an epidemic disease in the world, the treatment and prevention of obesity methods have gained great attention. Lactobacillus is the main member of probiotics, and the physiological activity of it is specific to different strains. This study systematically explored the anti-obesity effect and possible mechanism of Lactobacillus fermentum CQPC07 (LF-CQPC07), which was isolated from pickled vegetables. RESULTS: LF-CQPC07 effectively controlled the weight gain of mice caused by a high-fat diet. The results of pathological sections indicated that LF-CQPC07 alleviated hepatocyte damage and fat accumulation in adipocytes. The detection of biochemical indictors revealed that LF-CQPC07 decreased the levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG), and increased the level of high-density lipoprotein cholesterol (HDL-C). Additionally, LF-CQPC07 caused the decrease in the amounts of inflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-α (TNF-α), IL-6, and interferon-γ (IFN-γ), and the increase in the amounts of the anti-inflammatory cytokines IL-10 and IL-4. LF-CQPC07 also decreased the amounts of alanine aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP). Confirmed by qPCR, LF-CQPC07 enhanced the mRNA expression of catalase (CAT), gamma glutamylcysteine synthetase 1 (GSH1), copper/zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), and glutathione peroxidase (GSH-Px). It also increased the mRNA expression levels of carnitine palmitoyltransferase 1 (CPT1), peroxisome proliferator-activated receptor alpha (PPAR-α), lipoprotein lipase (LPL), and cholesterol 7 alpha hydroxylase (CYP7A1), and decreased that of PPAR-γ and CCAAT/enhancer binding protein alpha (C/EBP-α) in the liver of mice. CONCLUSION: This research confirmed that LF-CQPC07 is capable of ameliorating obesity, improving hyperlipemia, and alleviating chronic low-grade inflammation and liver injury accompanied with obesity. Its mechanism may be the regulation of antioxidant capacity and lipid metabolism. Therefore, LF-CQPC07 has enormous potential to serve as a potential probiotic for the prevention or treatment of obesity.
Authors: Peter J Turnbaugh; Ruth E Ley; Michael A Mahowald; Vincent Magrini; Elaine R Mardis; Jeffrey I Gordon Journal: Nature Date: 2006-12-21 Impact factor: 49.962
Authors: Buck S Samuel; Abdullah Shaito; Toshiyuki Motoike; Federico E Rey; Fredrik Backhed; Jill K Manchester; Robert E Hammer; S Clay Williams; Jan Crowley; Masashi Yanagisawa; Jeffrey I Gordon Journal: Proc Natl Acad Sci U S A Date: 2008-10-17 Impact factor: 11.205
Authors: Theofilos Poutahidis; Markus Kleinewietfeld; Christopher Smillie; Tatiana Levkovich; Alison Perrotta; Siddheshvar Bhela; Bernard J Varian; Yassin M Ibrahim; Jessica R Lakritz; Sean M Kearney; Antonis Chatzigiagkos; David A Hafler; Eric J Alm; Susan E Erdman Journal: PLoS One Date: 2013-07-10 Impact factor: 3.240