Haixiu Ma1, Kaizhen Yang2, Hongxia Li1, Mengyu Luo1, Reziwan Wufuer1, Ling Kang3. 1. College of Public Health, Xinjiang Medical University, Urumqi 830000, China. 2. Teaching & Research Department, The First People's Hospital of Urumqi, Urumqi 830000, China. 3. College of Public Health, Xinjiang Medical University, Urumqi 830000, China. Electronic address: 540802213@qq.com.
Abstract
BACKGROUND: Photodynamic therapy (PDT) is based on photochemical and photobiological reactions mediated by photosensitizers to achieve a killing effect on diseased cells. It is used in the treatment of malignant tumors, precancerous lesions and infections. OBJECTIVE: In order to provide theoretical data for further study of the mechanism of PDT for colorectal cancer, SW480 cells were treated with Ce6-PDT and effect of photodynamic therapy (Ce6-PDT) on cytoskeleton and E-cadherin protein were observed. METHODS: The survival of SW480 cells was detected by MTT assay. The morphological changes of SW480 cells after Ce6-PDT were observed by scanning electron microscope (ESM). The migration ability was determined by wound healing assay. The distribution of F-actin in the cytoplasm was observed with confocal laser scanning microscope. Western blot analysis was used to detect the expression of cytoskeleton proteins in SW480 cells after Ce6-PDT. RESULTS: Compared with the control group, there was significant difference in cell viability of cells treated with Ce6-PDT (F = 78753.78, P < 0.05). The pseudopodia almost disappeared and cellular atrophy was clearly visible in the cells of Ce6-PDT group. The migration ability of cells treated with Ce6-PDT for 48 h was significantly lower than the control group (F = 11.794, P<0.001). The result of Western blot analysis showed that the expression of F-actin, α-tubulin, β-tubulin and Vimentin in the cells treated with Ce6-PDT were significantly higher than that in the control group (F = 22.251,8.109, 5.840, 4.685 and 18.754, P < 0.05). The expression of E-cadherin in cells of Ce6-PDT group was significantly higher than that in control group (F = 30.882, P < 0.001). Perhaps Ce6-PDT inhibits the proliferation and migration of colon cancer SW480 cells by enhancing the expression of E-cadherin, causing the disappearance of cell pseudopodia and the destruction of cytoskeleton. CONCLUSIONS: The destruction of cytoskeleton might be one of the reasons for the inhibition of cell proliferation and migration by Ce6-PDT.
BACKGROUND: Photodynamic therapy (PDT) is based on photochemical and photobiological reactions mediated by photosensitizers to achieve a killing effect on diseased cells. It is used in the treatment of malignant tumors, precancerous lesions and infections. OBJECTIVE: In order to provide theoretical data for further study of the mechanism of PDT for colorectal cancer, SW480 cells were treated with Ce6-PDT and effect of photodynamic therapy (Ce6-PDT) on cytoskeleton and E-cadherin protein were observed. METHODS: The survival of SW480 cells was detected by MTT assay. The morphological changes of SW480 cells after Ce6-PDT were observed by scanning electron microscope (ESM). The migration ability was determined by wound healing assay. The distribution of F-actin in the cytoplasm was observed with confocal laser scanning microscope. Western blot analysis was used to detect the expression of cytoskeleton proteins in SW480 cells after Ce6-PDT. RESULTS: Compared with the control group, there was significant difference in cell viability of cells treated with Ce6-PDT (F = 78753.78, P < 0.05). The pseudopodia almost disappeared and cellular atrophy was clearly visible in the cells of Ce6-PDT group. The migration ability of cells treated with Ce6-PDT for 48 h was significantly lower than the control group (F = 11.794, P<0.001). The result of Western blot analysis showed that the expression of F-actin, α-tubulin, β-tubulin and Vimentin in the cells treated with Ce6-PDT were significantly higher than that in the control group (F = 22.251,8.109, 5.840, 4.685 and 18.754, P < 0.05). The expression of E-cadherin in cells of Ce6-PDT group was significantly higher than that in control group (F = 30.882, P < 0.001). Perhaps Ce6-PDT inhibits the proliferation and migration of colon cancerSW480 cells by enhancing the expression of E-cadherin, causing the disappearance of cell pseudopodia and the destruction of cytoskeleton. CONCLUSIONS: The destruction of cytoskeleton might be one of the reasons for the inhibition of cell proliferation and migration by Ce6-PDT.