Fatemeh Ramezanalizadeh1, Iraj Rasooli2, Parviz Owlia3. 1. Department of Biology, Shahed University, Tehran, 3319118651, Iran. 2. Department of Biology, Molecular Microbiology Research Center, Shahed University, Tehran, 3319118651, Iran. 3. Department of Microbiology, Molecular Microbiology Research Center, School of Medicine, Shahed University, Tehran, 3319118651, Iran.
Abstract
Aim: Iron uptake and metabolism pathways are promising targets in vaccine development as an alternative strategy for antibiotics. Methods & methods: HemTR, a putative heme receptor of Acinetobacter baumannii, was expressed and its protectivity against A. baumannii was determined singly or in combination with the siderophore receptor, BauA, in mice. Results: High level of IgG was elicited. There was a delay in mice mortality with reduced bacterial loads in internal organs in the sublethal challenge. Protection was better in the HemTR-BauA group in both lethal and sublethal challenges. Passive transfer of anti-HemTR and anti-BauA partially protected mice against A. baumannii infection. Conclusion: HemTR in combination with other iron receptors could contribute to the development of protective vaccines against A. baumannii.
Aim: Iron uptake and metabolism pathways are promising targets in vaccine development as an alternative strategy for antibiotics. Methods & methods: HemTR, a putative heme receptor of Acinetobacter baumannii, was expressed and its protectivity against A. baumannii was determined singly or in combination with the siderophore receptor, BauA, in mice. Results: High level of IgG was elicited. There was a delay in micemortality with reduced bacterial loads in internal organs in the sublethal challenge. Protection was better in the HemTR-BauA group in both lethal and sublethal challenges. Passive transfer of anti-HemTR and anti-BauA partially protected mice against A. baumannii infection. Conclusion:HemTR in combination with other iron receptors could contribute to the development of protective vaccines against A. baumannii.
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Keywords:
A. baumannii; BauA; HemTR; iron receptor; vaccine