Rafael B S Valadares1, Fabio Marroni2,3, Fabiano Sillo4, Renato R M Oliveira1,5, Raffaella Balestrini4, Silvia Perotto6. 1. Instituto Tecnológico Vale, Rua Boaventura da Silva 955, 66050-000 Belém, Pará, Brazil. 2. Dipartimento di Scienze Agroalimentari, Ambientali e Animali, Università di Udine, Via delle Scienze, I-33100 Udine, Italy. 3. Istituto di Genomica Applicata, Via Linussio 51, I-33100 Udine, Italy. 4. Consiglio Nazionale Delle Ricerche-Istituto per la Protezione Sostenibile Delle Piante, Viale P.A. Mattioli 25, I-10125 Torino, Italy. 5. Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos, 6627, 31270-901 Belo Horizonte, Minas Gerais, Brazil. 6. Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Viale Mattioli 25, I-10125 Torino, Italy.
Abstract
The study of orchid mycorrhizal interactions is particularly complex because of the peculiar life cycle of these plants and their diverse trophic strategies. Here, transcriptomics has been applied to investigate gene expression in the mycorrhizal roots of Limodorum abortivum, a terrestrial mixotrophic orchid that associates with ectomycorrhizal fungi in the genus Russula. Our results provide new insights into the mechanisms underlying plant-fungus interactions in adult orchids in nature and in particular into the plant responses to the mycorrhizal symbiont(s) in the roots of mixotrophic orchids. Our results indicate that amino acids may represent the main nitrogen source in mycorrhizal roots of L. abortivum, as already suggested for orchid protocorms and other orchid species. The upregulation, in mycorrhizal L. abortivum roots, of some symbiotic molecular marker genes identified in mycorrhizal roots from other orchids as well as in arbuscular mycorrhiza, may mirror a common core of plant genes involved in endomycorrhizal symbioses. Further efforts will be required to understand whether the specificities of orchid mycorrhiza depend on fine-tuned regulation of these common components, or whether specific additional genes are involved.
The study of orchid mycorrhizal interactions is particularly complex because of the peculiar life cycle of these plants and their diverse trophic strategies. Here, transcriptomics has been applied to investigate gene expression in the mycorrhizal roots of n class="Species">Limodorum abortivum, a terrestrial mixotrophic orchid that associates with ectomycorrhizal fungi inpan> the genus pan> class="Species">Russula. Our results provide new insights into the mechanisms underlying plant-fungus interactions in adult orchids in nature and in particular into the plant responses to the mycorrhizal symbiont(s) in the roots of mixotrophic orchids. Our results indicate that amino acids may represent the main nitrogen source in mycorrhizal roots of L. abortivum, as already suggested for orchid protocorms and other orchid species. The upregulation, in mycorrhizal L. abortivum roots, of some symbiotic molecular marker genes identified in mycorrhizal roots from other orchids as well as in arbuscular mycorrhiza, may mirror a common core of plant genes involved in endomycorrhizal symbioses. Further efforts will be required to understand whether the specificities of orchid mycorrhiza depend on fine-tuned regulation of these common components, or whether specific additional genes are involved.
Authors: Mike Guether; Raffaella Balestrini; Matthew Hannah; Ji He; Michael K Udvardi; Paola Bonfante Journal: New Phytol Date: 2009-01-13 Impact factor: 10.151
Authors: Paul Bayman; Ana T Mosquera-Espinosa; Carla M Saladini-Aponte; Nilbeth C Hurtado-Guevara; Naida L Viera-Ruiz Journal: Am J Bot Date: 2016-10-26 Impact factor: 3.844