Alexander P Hertle1, Benedikt Haberl1, Ralph Bock2. 1. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam-Golm, Germany. 2. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam-Golm, Germany. rbock@mpimp-golm.mpg.de.
Abstract
Recent work has revealed that both plants and animals transfer genomes between cells. In plants, horizontal transfer of entire plastid, mitochondrial, or nuclear genomes between species generates new combinations of nuclear and organellar genomes, or produces novel species that are allopolyploid. The mechanisms of genome transfer between cells are unknown. Here, we used grafting to identify the mechanisms involved in plastid genome transfer from plant to plant. We show that during proliferation of wound-induced callus, plastids dedifferentiate into small, highly motile, amoeboid organelles. Simultaneously, new intercellular connections emerge by localized cell wall disintegration, forming connective pores through which amoeboid plastids move into neighboring cells. Our work uncovers a pathway of organelle movement from cell to cell and provides a mechanistic framework for horizontal genome transfer.
Recent work has revealed that both plants and animals transfer genomes between cells. In plants, horizontal transfer of entire plastid, mitochondrial, or nuclear genomes between species generates new combinations of nuclear and organellar genomes, or produces novel species that are allopolyploid. The mechanisms of genome transfer between cells are unknown. Here, we used grafting to identify the mechanisms involved in plastid genome transfer from plant to plant. We show that during proliferation of wound-induced callus, plastids dedifferentiate into small, highly motile, amoeboid organelles. Simultaneously, new intercellular connections emerge by localized cell wall disintegration, forming connective pores through which amoeboid plastids move into neighboring cells. Our work uncovers a pathway of organelle movement from cell to cell and provides a mechanistic framework for horizontal genome transfer.
Authors: An S Tan; James W Baty; Lan-Feng Dong; Ayenachew Bezawork-Geleta; Berwini Endaya; Jacob Goodwin; Martina Bajzikova; Jaromira Kovarova; Martin Peterka; Bing Yan; Elham Alizadeh Pesdar; Margarita Sobol; Anatolyj Filimonenko; Shani Stuart; Magdalena Vondrusova; Katarina Kluckova; Karishma Sachaphibulkij; Jakub Rohlena; Pavel Hozak; Jaroslav Truksa; David Eccles; Larisa M Haupt; Lyn R Griffiths; Jiri Neuzil; Michael V Berridge Journal: Cell Metab Date: 2015-01-06 Impact factor: 27.287