Emrah Özsoy1, Musab Ali Kutluhan2, Mehmet Akyüz3, Emre Tokuç4, Ahmet Ürkmez5, Gülistan Gümrükçü3, Metin İshak Öztürk3. 1. Unye State Hospital, Yuceler Mh. Kayalar Sk. No:3, Ordu, Turkey. emrahozsoy@gmail.com. 2. Department of Urology, Yildirim Beyazit University School of Medicine, Ayvalı Mh. Gazze Cd. No: 7, Etlik-Keçiören, Ankara, Turkey. 3. Department of Urology, Health Sciences University Haydarpasa Numune Training and Research Hospital, Tıbbıye Cad. No:23, Tr-34668, Istanbul, Turkey. 4. Sultanbeyli State Hospital, Battalgazi Mh Paşaköy Cd. No:60, 34935, Sultanbeyli, Istanbul, Turkey. 5. Department of Urology, University of Texas MD Anderson Cancer Center, 4002, 1515 Holcombe Blvd, Houston, TX, 77030, USA.
Abstract
PURPOSE: To investigate the effect of platelet-rich plasma (PRP) in reducing renal injury in ureteral obstruction. METHODS: Twenty-four Wistar Albino rats were randomized and divided into four groups as the donor (n = 6), sham (n = 6), saline (n = 6), and PRP (n = 6). Blood was obtained from the donor group by cardiac puncture and PRP was prepared. 2 cc blood was sampled from other groups to measure blood-urea nitrogen and creatinine levels. Baseline renal scintigraphy was performed. An abdominal midline incision was made and the left ureter was exposed in the sham group. Saline infusion was given to the kidneys of the saline group after left ureteral obstruction, while PRP was given to the PRP group. On postoperative Day 7, control biochemical and scintigraphic evaluations were performed and left nephrectomies were done. Left kidneys were evaluated histopathologically. RESULTS: DMSA measurements in the sham group were found to be significantly higher than the saline and PRP groups (p = 0.001 and p = 0.024, respectively). There were no significant differences between the saline and PRP groups (p = 0.525 and p > 0.05, respectively). Histopathologically, no significant difference was observed between the saline and PRP groups (p = 0.320), while the scores of the sham group were significantly higher than the saline and PRP groups (p = 0.02 and p = 0.001, respectively). CONCLUSION: Our study results suggest that PRP may be effective in preventing ureteral obstruction-induced renal injury.
PURPOSE: To investigate the effect of platelet-rich plasma (PRP) in reducing renal injury in ureteral obstruction. METHODS: Twenty-four Wistar Albino rats were randomized and divided into four groups as the donor (n = 6), sham (n = 6), saline (n = 6), and PRP (n = 6). Blood was obtained from the donor group by cardiac puncture and PRP was prepared. 2 cc blood was sampled from other groups to measure blood-ureanitrogen and creatinine levels. Baseline renal scintigraphy was performed. An abdominal midline incision was made and the left ureter was exposed in the sham group. Saline infusion was given to the kidneys of the saline group after left ureteral obstruction, while PRP was given to the PRP group. On postoperative Day 7, control biochemical and scintigraphic evaluations were performed and left nephrectomies were done. Left kidneys were evaluated histopathologically. RESULTS:DMSA measurements in the sham group were found to be significantly higher than the saline and PRP groups (p = 0.001 and p = 0.024, respectively). There were no significant differences between the saline and PRP groups (p = 0.525 and p > 0.05, respectively). Histopathologically, no significant difference was observed between the saline and PRP groups (p = 0.320), while the scores of the sham group were significantly higher than the saline and PRP groups (p = 0.02 and p = 0.001, respectively). CONCLUSION: Our study results suggest that PRP may be effective in preventing ureteral obstruction-induced renal injury.
Authors: Alvaro C Ucero; Alberto Benito-Martin; Maria C Izquierdo; Maria D Sanchez-Niño; Ana B Sanz; Adrian M Ramos; Sergio Berzal; Marta Ruiz-Ortega; Jesus Egido; Alberto Ortiz Journal: Int Urol Nephrol Date: 2013-09-27 Impact factor: 2.370