Literature DB >> 33512617

Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA.

Ute Eberle1, Clara Wimmer2, Nikolaus Ackermann1, Andreas Sing3,4, Ingrid Huber5, Antonie Neubauer-Juric6, Giuseppe Valenza7.   

Abstract

To face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating nine commercially available real-time RT-PCR assays. We found that assays differed considerably in performance and validation before routine use is mandatory.

Entities:  

Keywords:  COVID-19; Coronavirus; Diagnostics; Infectious disease; Laboratory diagnostics; Molecular testing; Real-time PCR; SARS-CoV-2

Year:  2021        PMID： 33512617      PMCID： PMC7845288          DOI： 10.1007/s10096-021-04159-9

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  6 in total

1.  The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes.

Authors:  Roberto Luraschi; Carlos Barrera-Avalos; Eva Vallejos-Vidal; Javiera Alarcón; Andrea Mella-Torres; Felipe Hernández; Ailen Inostroza-Molina; Daniel Valdés; Mónica Imarai; Claudio Acuña-Castillo; Felipe E Reyes-López; Ana María Sandino
Journal:  Can J Infect Dis Med Microbiol       Date:  2022-07-05       Impact factor: 2.585

2.  Comparative performance of CDC-modified SARS-CoV-2 real-time PCR assay with four different commercial assays: laboratory-based study.

Authors:  Dayakar Seetha; Amjesh Ravikumar; Radhakrishnan R Nair
Journal:  Comp Clin Path       Date:  2022-05-26

3.  Analysis of seven SARS-CoV-2 rapid antigen tests in detecting omicron (B.1.1.529) versus delta (B.1.617.2) using cell culture supernatants and clinical specimens.

Authors:  Sabrina Jungnick; Bernhard Hobmaier; Natali Paravinja; Lena Mautner; Mona Hoyos; Regina Konrad; Maren Haase; Armin Baiker; Ute Eberle; Magdalena Bichler; Bianca Treis; Mercy Okeyo; Barbara Streibl; Clara Wimmer; Sabrina Hepner; Annika Sprenger; Carola Berger; Laura Weise; Alexandra Dangel; Siegfried Ippisch; Walter Jonas; Manfred Wildner; Bernhard Liebl; Nikolaus Ackermann; Andreas Sing; Volker Fingerle
Journal:  Infection       Date:  2022-05-20       Impact factor: 7.455

4.  Detection of the new SARS-CoV-2 variants of concern B.1.1.7 and B.1.351 in five SARS-CoV-2 rapid antigen tests (RATs), Germany, March 2021.

Authors:  Sabrina Jungnick; Bernhard Hobmaier; Lena Mautner; Mona Hoyos; Maren Haase; Armin Baiker; Heidi Lahne; Ute Eberle; Clara Wimmer; Sabrina Hepner; Annika Sprenger; Carola Berger; Alexandra Dangel; Manfred Wildner; Bernhard Liebl; Nikolaus Ackermann; Andreas Sing; Volker Fingerle
Journal:  Euro Surveill       Date:  2021-04

5.  Virological COVID-19 surveillance in Bavaria, Germany suggests no SARS-CoV-2 spread prior to the first German case in January 2020.

Authors:  Ute Eberle; Susanne Heinzinger; Nikolaus Ackermann; Andreas Sing; Regina Konrad; Clara Wimmer; Bernhard Liebl; Katharina Katz
Journal:  Infection       Date:  2021-04-23       Impact factor: 3.553

6.  Problems associated with mass border testing of COVID-19.

Authors:  Mathias Grunér; Marika Nordberg; Knut Lönnroth
Journal:  Scand J Public Health       Date:  2021-06-19       Impact factor: 3.021
  6 in total

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