Ana Fuentes1, Esther Serrano-Conde1, Carolina Roldán2, Rafael Benito-Ruesca3, Gregoria Mejías4, Antonio Sampedro5, Gabriel March-Roselló6, Isabel Fernández-Natal7, Juliana Esperalba8, Mario José Rodríguez9, Paula Martínez de Aguirre10, Carlos Salas11, María Lourdes Roc12, Luis Miguel Soria13, Mónica Parra-Grande14, María Dolores Montero15, Ricardo Fernández-Roblas16, Francisco Franco-Álvarez de Luna17, Carmen Lozano18, Federico García19. 1. Hospital Universitario Clínico San Cecilio, Instituto de Investigación Ibs, Av, Innovación S/N, 18016, Granada, Spain. 2. Complejo Hospitalario de Jaén, Jaén, Spain. 3. Hospital Clínico Universitario Lozano Blesa, Zaragoza, Spain. 4. Hospital Universitario de Burgos, Burgos, Spain. 5. Hospital Universitario Virgen de las Nieves, Granada, Spain. 6. Hospital Clínico Universitario de Valladolid, Valladolid, Spain. 7. Complejo Asistencial Universitario de León, León, Spain. 8. Hospital Universitario Valle de Hebrón, Barcelona, Spain. 9. Hospital Ramón y Cajal, Madrid, Spain. 10. Clínica Universidad de Navarra, Pamplona, Spain. 11. Hospital Universitario Marqués de Valdecilla, Santander, Spain. 12. Hospital Universitario Miguel Servet, Zaragoza, Spain. 13. Hospital San Pedro, Logroño, Spain. 14. Complejo Hospitalario Universitario de Albacete, Albacete, Spain. 15. Hospital Universitario La Paz, Madrid, Spain. 16. Hospital Universitario Fundación Jiménez Díaz, Madrid, Spain. 17. Hospital Universitario Juan Ramón Jiménez, Huelva, Spain. 18. Hospital Universitario Virgen del Rocío, Sevilla, Spain. 19. Hospital Universitario Clínico San Cecilio, Instituto de Investigación Ibs, Av, Innovación S/N, 18016, Granada, Spain. fegarcia@ugr.es.
Abstract
AIM: To evaluate the serological response against SARS-CoV-2 in a multicenter study representative of the Spanish COVID pandemic. METHODS: IgG and IgM + IgA responses were measured on 1466 samples from 1236 Spanish COVID-19 patients admitted to the hospital, two commercial ELISA kits (Vircell SL, Spain) based on the detection of antibodies against the viral spike protein and nucleoprotein, were used. RESULTS: Approximately half of the patients presented antibodies (56.8% were IgM + IgA positive and 43.0% were IgG positive) as soon as 2 days after the first positive PCR result. Serological test positivity increased with time from the PCR test, and 10 days after the first PCR result, 91.5% and 88.0% of the patients presented IgM + IgA and IgG antibodies, respectively. CONCLUSION: The high values of sensitivity attained in the present study from a relatively early period of time after hospitalization support the use of the evaluated serological assays as supplementary diagnostic tests for the clinical management of COVID-19.
AIM: To evaluate the serological response against SARS-CoV-2 in a multicenter study representative of the Spanish COVID pandemic. METHODS: IgG and IgM + IgA responses were measured on 1466 samples from 1236 Spanish COVID-19patients admitted to the hospital, two commercial ELISA kits (Vircell SL, Spain) based on the detection of antibodies against the viral spike protein and nucleoprotein, were used. RESULTS: Approximately half of the patients presented antibodies (56.8% were IgM + IgA positive and 43.0% were IgG positive) as soon as 2 days after the first positive PCR result. Serological test positivity increased with time from the PCR test, and 10 days after the first PCR result, 91.5% and 88.0% of the patients presented IgM + IgA and IgG antibodies, respectively. CONCLUSION: The high values of sensitivity attained in the present study from a relatively early period of time after hospitalization support the use of the evaluated serological assays as supplementary diagnostic tests for the clinical management of COVID-19.
Authors: Victor M Corman; Ali M Albarrak; Ali Senosi Omrani; Mohammed M Albarrak; Mohamed Elamin Farah; Malak Almasri; Doreen Muth; Andrea Sieberg; Benjamin Meyer; Abdullah M Assiri; Tabea Binger; Katja Steinhagen; Erik Lattwein; Jaffar Al-Tawfiq; Marcel A Müller; Christian Drosten; Ziad A Memish Journal: Clin Infect Dis Date: 2015-11-12 Impact factor: 9.079
Authors: Gabriella S Oliveira; Luciana Silva-Flannery; Juliana F Silva; Charlene Siza; Roberto J Esteves; Barbara J Marston; Juliette Morgan; Mateusz Plucinski; Tárcio P Roca; Ana M P Silva; Soraya S Pereira; Juan M V Salcedo; Dhelio B Pereira; Felipe G Naveca; Deusilene S Vieira Dall'Acqua Journal: J Med Virol Date: 2022-03-22 Impact factor: 20.693