Davide B Gissi1, Luca Morandi2, Giuseppe Colella3, Roberto De Luca3, Giuseppina Campisi4, Rodolfo Mauceri4, Umberto Romeo5, Gianluca Tenore5, Michele D Mignogna6, Daniela Adamo6, Giacomo Oteri7, Antonia Marcianò7, Paolo Vescovi8, Marco Meleti8, Rosario Serpico3, Dario Di Stasio3, Monica Pentenero9, Andrea Santarelli10, Alberto Bianchi11, Salvatore Crimi11, Claudio Marchetti12, Achille Tarsitano12, Maria Pia Foschini13, Lucio Montebugnoli1. 1. Department of Biomedical and Neuromotor Sciences, Section of Oral Sciences, University of Bologna, Bologna, Italy. 2. Functional MR Unit, IRCCS Istituto delle Scienze Neurologiche di Bologna, Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy. 3. Multidisciplinary Department of Medical-Surgical and Dental Specialities, University of Campania "L. Vanvitelli", Naples, Italy. 4. Department of Surgical, Oncological, and oral Sciences, Sector of Oral Medicine "Valerio Margiotta", University of Palermo, Palermo, Italy. 5. Department of Oral and Maxillofacial Sciences, "Sapienza" University of Rome, Rome, Italy. 6. Oral Medicine Complex Unit, Department of Neurosciences, Reproductive and Odontostomatological Sciences - Head & Neck Clinical Section, "Federico II" University of Naples, Naples, Italy. 7. Department of Biomedical, Dental Sciences and Morphofunctional Imaging, University of Messina, Messina, Italy. 8. Oral Medicine and Laser Surgery Unit, Department of Medicine and Surgery, University of Parma, Parma, Italy. 9. Oral Medicine and Oral Oncology Unit, Department of Oncology, University of Turin, Turin, Italy. 10. Department of Clinical Sciences and Stomatology, Marche Polytechnic University, Ancona, Italy. 11. Section of Maxillo-Facial Surgery, Department of General Surgery, Policlinico - San Marco, University of Catania, Catania, Italy. 12. Section of Maxillo-Facial Surgery at Policlinico S. Orsola-Malpighi, Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy. 13. Section of Anatomic Pathology at Bellaria Hospital, Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
Abstract
BACKGROUND: The aim of this Italian multicenter study was to evaluate the diagnostic performance of a minimally invasive method for the detection of oral squamous cell carcinoma (OSCC) based on 13-gene DNA methylation analysis in oral brushing samples. METHODS: Oral brushing specimens were collected in 11 oral medicine centers across Italy. Twenty brushing specimens were collected by each center, 10 from patients with OSCC, and 10 from healthy volunteers. DNA methylation analysis was performed in blindness, and each sample was determined as positive or negative based on a predefined cutoff value. RESULTS: DNA amplification failed in 4 of 220 (1.8%) samples. Of the specimens derived from patients with OSCC, 93.6% (103/110) were detected as positive, and 84.9% (90/106) of the samples from healthy volunteers were negative. CONCLUSION: These data confirmed the diagnostic performance of our novel procedure in a large cohort of brushing specimens collected from 11 different centers and analyzed in blindness.
BACKGROUND: The aim of this Italian multicenter study was to evaluate the diagnostic performance of a minimally invasive method for the detection of oral squamous cell carcinoma (OSCC) based on 13-gene DNA methylation analysis in oral brushing samples. METHODS: Oral brushing specimens were collected in 11 oral medicine centers across Italy. Twenty brushing specimens were collected by each center, 10 from patients with OSCC, and 10 from healthy volunteers. DNA methylation analysis was performed in blindness, and each sample was determined as positive or negative based on a predefined cutoff value. RESULTS: DNA amplification failed in 4 of 220 (1.8%) samples. Of the specimens derived from patients with OSCC, 93.6% (103/110) were detected as positive, and 84.9% (90/106) of the samples from healthy volunteers were negative. CONCLUSION: These data confirmed the diagnostic performance of our novel procedure in a large cohort of brushing specimens collected from 11 different centers and analyzed in blindness.
Authors: Chi T Viet; Xinyu Zhang; Ke Xu; Gary Yu; Kesava Asam; Carissa M Thomas; Nicholas F Callahan; Coleen Doan; Paul C Walker; Khanh Nguyen; Stephanie C Kidd; Steve C Lee; Anupama Grandhi; Clint T Allen; Simon Young; James C Melville; Jonathan W Shum; Dan T Viet; Alan S Herford; Dylan F Roden; Manuel L Gonzalez; Jiang F Zhong; Bradley E Aouizerat Journal: Biomark Res Date: 2021-12-20
Authors: Julianna K Bronk; Chiraag Kapadia; Xiaogang Wu; Bhavana V Chapman; Rui Wang; Tatiana V Karpinets; Xingzhi Song; Andrew M Futreal; Jianhua Zhang; Ann H Klopp; Lauren E Colbert Journal: PLoS One Date: 2022-10-06 Impact factor: 3.752