Donggang Mou1, Qunying Yu2, Jimei Zhang3, Jianping Zhou4, Xinmin Li4, Weiyi Zhuang5, Xuming Yang6. 1. Department of Orthopedics, Yan'an Hospital Affiliated to Kunming Medical University, Kunming, 650035, People's Republic of China. 2. Department of Maternity, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650051, People's Republic of China. 3. Department of Gastroenterology, Chenggong Hospital, Yan'an Hospital Affiliated to Kunming Medical University, Kunming, 650035, People's Republic of China. 4. Department of Orthopedics, Chenggong Hospital, Yan'an Hospital Affiliated to Kunming Medical University, Kunming, 650035, People's Republic of China. 5. Department of Cardiology, Chenggong Hospital, Yan'an Hospital Affiliated to Kunming Medical University, Kunming, 650035, People's Republic of China. 6. Department of Orthopedics, Chenggong Hospital, Yan'an Hospital Affiliated to Kunming Medical University, Kunming, 650035, People's Republic of China. xming71@126.com.
Abstract
BACKGROUND: Pain and cartilage destruction caused by osteoarthritis (OA) is a major challenge in clinical treatment. Traditional intra-articular injection of hyaluronic acid (HA) can relieve the disease, but limited by the difficulty of long-term maintenance of efficacy. METHODS: In this study, an injectable and self-healing hydrogel was synthesized by in situ crosslinking of N-carboxyethyl chitosan (N-chitosan), adipic acid dihydrazide (ADH), and hyaluronic acid-aldehyde (HA-ALD). RESULTS: This supramolecular hydrogel sustains good biocompatibility for chondrocytes. Intra-articular injection of this novel hydrogel can significantly alleviate the local inflammation microenvironment in knee joints, through inhibiting the inflammatory cytokines (such as TNF-α, IL-1β, IL-6 and IL-17) in the synovial fluid and cartilage at 2- and even 12-weeks post-injection. Histological and behavioral test indicated that hydrogel injection protected cartilage destruction and relieved pain in OA rats, in comparison to HA injection. CONCLUSION: This kind of novel hydrogel, which is superior to the traditional HA injection, reveals a great potential for the treatment of OA.
BACKGROUND:Pain and cartilage destruction caused by osteoarthritis (OA) is a major challenge in clinical treatment. Traditional intra-articular injection of hyaluronic acid (HA) can relieve the disease, but limited by the difficulty of long-term maintenance of efficacy. METHODS: In this study, an injectable and self-healing hydrogel was synthesized by in situ crosslinking of N-carboxyethyl chitosan (N-chitosan), adipic acid dihydrazide (ADH), and hyaluronic acid-aldehyde (HA-ALD). RESULTS: This supramolecular hydrogel sustains good biocompatibility for chondrocytes. Intra-articular injection of this novel hydrogel can significantly alleviate the local inflammation microenvironment in knee joints, through inhibiting the inflammatory cytokines (such as TNF-α, IL-1β, IL-6 and IL-17) in the synovial fluid and cartilage at 2- and even 12-weeks post-injection. Histological and behavioral test indicated that hydrogel injection protected cartilage destruction and relieved pain in OA rats, in comparison to HA injection. CONCLUSION: This kind of novel hydrogel, which is superior to the traditional HA injection, reveals a great potential for the treatment of OA.
Authors: Mira Meeus; Stijn Vervisch; Luc S De Clerck; Greta Moorkens; Guy Hans; Jo Nijs Journal: Semin Arthritis Rheum Date: 2011-10-28 Impact factor: 5.532
Authors: Piotr Bełdowski; Maciej Przybyłek; Alina Sionkowska; Piotr Cysewski; Magdalena Gadomska; Katarzyna Musiał; Adam Gadomski Journal: Materials (Basel) Date: 2022-01-08 Impact factor: 3.623